Are changes in membrane lipids and fatty acid composition related to salt-stress resistance in wild and cultivated barley?
Are changes in membrane lipids and fatty acid composition related to salt-stress resistance in wild and cultivated barley?
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DOI：
10.1002/jpln.201100413
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发表时间：
2013-04-01
影响因子：
2.5
通讯作者：
Ben Youssef, Nabil
中科院分区：
文献类型：
--
作者：
Chalbi, Najla;Hessini, Kamel;Ben Youssef, Nabil
研究了利用膜脂测定筛选大麦耐盐基因型的可能性。结果表明，与栽培大麦相比，野生大麦表现出典型的盐生反应。CV. Manel）。生长、组织水合和光合活性受盐度影响较小。maritimum比H.粗俗的。长期NaCl处理的诱导效应反映在野生大麦根膜脂保持相对不变，而它们显着减少，随着盐浓度的增加，在H。粗俗。膜脂过氧化和电解质渗漏的水平只在高盐浓度下才发生变化。maritimum，而H.由于氧化损伤，低盐度水平显著增加了vulgare。这些结果表明，维持膜完整性（在H。Maritimum）可能被认为是抗盐性的一个可能性状。而H. vulgare比H.海洋。因此，不饱和脂肪酸与饱和脂肪酸之比（UFAs：SFAs）和双键指数（DBI）在盐胁迫下显著增加，而大麦则没有变化。海洋。脂质不饱和度的变化主要是由于亚麻酸（C18：3）、亚油酸（C18：2）和油酸（C18：1）的增加以及硬脂酸（C18：0）的减少。这些结果表明，尽管是重要的膜流动性的维持，增加不饱和度的能力是不是一个决定性因素，大麦品种的耐盐性。
The possibility to use membrane-lipid measurements to screen barley genotypes for salt resistance was studied. The results showed that wild barley (Hordeum maritimum) displayed a typical halophytic response as compared to cultivated barley (Hordeum vulgare L. cv. Manel). Growth, tissue hydration, and photosynthetic activity were less affected by salinity in H. maritimum than in H. vulgare. The induced effects of long-term NaCl treatment were reflected in root membrane lipids that remained relatively unchanged in wild barley, whilst they were significantly diminished with increasing salinity in H. vulgare. The levels of membrane-lipid peroxidation and electrolyte leakage were changed only at high salt concentrations in H. maritimum whereas those of H. vulgare were considerably increased by lower salinity levels as a result of oxidative damage. These findings indicate that maintained membrane integrity (in H. Maritimum) may be considered a possible trait for salt resistance. However, membrane fluidity in H. vulgare was more increased than in H. maritimum. Thus, the unsaturatedtosaturated fatty acid ratio (UFAs : SFAs) and the double-bond index (DBI), significantly increased in response to salt stress in cultivated barley while it did not change in H. maritimum. The changes in lipid unsaturation were predominantly due to increases in linolenic (C18:3), linoleic (C18:2), and oleic (C18:1) acids and decreases in stearic acid (C18:0). These results suggest that, in spite of being important for maintenance of membrane fluidity, the ability to increase unsaturation is not a determinant factor for salt resistance in barley species.