How do bacteria tune translation efficiency?
How do bacteria tune translation efficiency?
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DOI：
10.1016/j.mib.2015.01.001
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发表时间：
2015-04
影响因子：
5.4
通讯作者：
Li GW
中科院分区：
文献类型：
--
作者：
Li GW
细菌蛋白质以精确确定的速率翻译，以满足细胞需求。相比之下，在细菌中表达重组蛋白的努力往往在其翻译水平上遇到很大的不可预测性。天然和合成mRNA的翻译之间的脱节源于对细菌用来调整翻译效率的策略缺乏了解。基于阵列的寡核苷酸合成和核糖体图谱的发展为解决这一问题提供了新的方法。尽管翻译效率的主要决定因素尚不清楚，但这些高通量研究指出，起始密码子周围的mRNA二级结构对翻译效率的贡献在统计学上是显著的，但影响很小。在这里，我总结了这些发现，并为衡量翻译效率提供了一个理论框架。
Bacterial proteins are translated with precisely determined rates to meet cellular demand. In contrast, efforts to express recombinant proteins in bacteria are often met with large unpredictability in their levels of translation. The disconnect between translation of natural and synthetic mRNA stems from the lack of understanding of the strategy used by bacteria to tune translation efficiency. The development of array-based oligonucleotide synthesis and ribosome profiling provides new approaches to address this issue. Although the major determinant for translation efficiency is still unknown, these high-throughput studies point out a statistically significant but mild contribution from the mRNA secondary structure around the start codon. Here I summarize those findings and provide a theoretical framework for measuring translation efficiency.