Localization of a putative tumor suppressor gene by using homozygous deletions in melanomas.
Localization of a putative tumor suppressor gene by using homozygous deletions in melanomas.
复制标题
通过使用黑色素瘤中的纯合缺失来定位推定的肿瘤抑制基因。
DOI：
10.1073/pnas.91.16.7563
复制
发表时间：
1994
影响因子：
11.1
通讯作者：
Kamb,A
中科院分区：
文献类型：
--
作者：
Weaver-Feldhaus,J;Gruis,NA;Neuhausen,S;LePaslier,D;Stockert,E;Skolnick,MH;Kamb,A
人们认为人类 9 号染色体的 p21 区域包含一个与黑色素瘤遗传易感性有关的基因 (MLM) 以及一个或多个影响某些其他肿瘤进展的基因。跨越 9p21 中推定肿瘤抑制基因周围大片区域的基因组克隆已被分离出来。该区域的一组序列标记位点已经开发出来。通过使用这些标记和先前报道的其他标记，已经通过物理作图在 84 个黑色素瘤细胞系中研究了 9p21 区域。假定的肿瘤抑制基因（可能是 MLM 本身）已定位于 α-干扰素基因簇近端（着丝粒）小于 40 kb 的区域。
The p21 region of human chromosome 9 is thought to contain a gene (MLM) involved in genetic susceptibility to melanoma and a gene or genes that influence progression of certain other tumors. Genomic clones that span a large region in 9p21 surrounding the presumptive tumor suppressor gene(s) have been isolated. A set of sequence-tagged sites in this region has been developed. By using these markers and others previously reported, the 9p21 region has been studied by physical mapping in 84 melanoma cell lines. A putative tumor suppressor gene, perhaps MLM itself, has been localized to a region of less than 40 kb that lies proximal (centromeric) to the alpha-interferon gene cluster.