Origin of magnetosome membrane: Proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane
Origin of magnetosome membrane: Proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane
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DOI：
10.1002/pmic.200500887
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发表时间：
2006-10-01
期刊：
影响因子：
3.4
通讯作者：
Matsunaga, Tadashi
中科院分区：
文献类型：
--
作者：
Tanaka, Masayoshi;Okamura, Yoshiko;Matsunaga, Tadashi
已知原核生物进化出一个或多个独特的细胞器。虽然关于这些细胞内成分的生物发生已经提出了几个假说，但这些建议中的大多数仍然不清楚。趋磁细菌合成被脂质双层膜包裹的细胞内磁小体。通过对几种表面和跨膜磁小体蛋白的鉴定和鉴定，我们推测磁小体来源于细胞质膜(CM)。为了证实这一假说，我们对趋磁细菌磁螺杆菌AMB-1的磁小体膜(MM)和磁小体CM进行了比较蛋白质组学分析。根据磁链霉菌AMB-1的全基因组序列，还发现78种MM蛋白在CM中普遍存在，其中一些与磁小体的生物合成有关，如紧密结合在磁铁矿表面的Mms13。还进行了脂肪酸分析，显示CM和MM轮廓之间有惊人的相似性。这些结果表明，MM是由CM派生的。
Prokaryotes are known to have evolved one or more unique organelles. Although several hypotheses have been proposed concerning the biogenesis of these intracellular components, the majority of these proposals remains unclear. Magnetotactic bacteria synthesize intracellular magnetosomes that are enclosed by lipid bilayer membranes. From the identification and characterization of several surface and transmembrane magnetosome proteins, we have postulated that magnetosomes are derived from the cytoplasmic membrane (CM). To confirm this hypothesis, a comparative proteomic analysis of the magnetosome membrane (MM) and CM of the magnetotactic bacterium, Magnetospirillum magneticum AMB-1, was undertaken. Based on the whole genome sequence of M. magneticum AMB-1, 78 identified MM proteins were also found to be prevalent in the CM, several of which are related to magnetosome biosynthesis, such as Mms13, which is tightly bound on the magnetite surface. Fatty acid analysis was also conducted, and showed a striking similarity between the CM and MM profiles. These results suggest that the MM is derived from the CM.