MOT1, A GLOBAL REPRESSOR OF RNA-POLYMERASE-II TRANSCRIPTION, INHIBITS TBP BINDING TO DNA BY AN ATP-DEPENDENT MECHANISM
MOT1, A GLOBAL REPRESSOR OF RNA-POLYMERASE-II TRANSCRIPTION, INHIBITS TBP BINDING TO DNA BY AN ATP-DEPENDENT MECHANISM
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DOI：
10.1101/gad.8.16.1920
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发表时间：
1994-08-15
影响因子：
10.5
通讯作者：
HAHN, S
中科院分区：
文献类型：
--
作者：
AUBLE, DT;HANSEN, KE;HAHN, S
在酵母中许多基因的基础转录被Mot 1抑制，Mot 1是一种必需蛋白，其是保守核因子的Snf 2/Swi 2家族的成员。ADI是TATA结合蛋白（TBP）与DNA结合的ATP依赖性抑制剂，在体外抑制转录。在这里，我们证明，ADI是由MOT 1基因编码。MOT 1的突变在体外和体内消除ADI活性并解除基础转录的抑制。重组Motl从DNA中去除TBP，并且Motl含有对其功能至关重要的ATP酶活性。Mot 1和TBP之间的遗传相互作用表明它们的功能在体内是相互关联的。这些结果提供了一个通用的模型，了解一个大家庭的核因子参与的过程，如转录和DNA修复的作用机制。.
Basal transcription of many genes in yeast is repressed by Mot1, an essential protein which is a member of the Snf2/Swi2 family of conserved nuclear factors. ADI is an ATP dependent inhibitor of TATA binding protein (TBP) binding to DNA that inhibits transcription in vitro. Here we demonstrate that ADI is encoded by the MOT1 gene. Mutation of MOT1 abolishes ADI activity and derepresses basal transcription in vitro and in vivo. Recombinant Motl removes TBP from DNA and Motl contains an ATPase activity which is essential for its function. Genetic interactions between Mot1 and TBP indicate that their functions are interlinked in vivo. These results provide a general model for understanding the mechanism of action of a large family of nuclear factors involved in processes such as transcription and DNA repair. .