DNA identification of Pacific bluefin tuna (Thunnus orientalis) in the New Zealand fishery
DNA identification of Pacific bluefin tuna (Thunnus orientalis) in the New Zealand fishery
复制标题
新西兰渔业中太平洋蓝鳍金枪鱼（Thunnus orientalis）的 DNA 鉴定
DOI：
10.1080/00288330.2001.9517046
复制
发表时间：
2001
影响因子：
1.6
通讯作者：
S. Chow
中科院分区：
文献类型：
--
作者：
P. J. Smith;L. Griggs;S. Chow
摘要1990 - 2000年在新西兰专属经济区（EEZ）采集了69条经鉴定为太平洋蓝鳍金枪鱼（Thunnus orientalis） （Temminck and Schlegel, 1844）的肌肉样本。1996年以前的鉴定是基于体型和尾骨的颜色；后来的鉴定主要是根据腹腔的形状。用一种诊断线粒体DNA标记对组织样本进行检测，该标记可区分南方蓝鳍金枪鱼（Thunnus maccoyii, Castelnau, 1872）和太平洋蓝鳍金枪鱼（T. orientalis）；经鉴定为东方绦虫59份，麦考伊绦虫10份。根据腹腔形状记录的太平洋蓝鳍金枪鱼标本被正确地鉴定为东方鳍金枪鱼，这一特征可用于鉴定落在金枪鱼船上的大型标本。一些根据颜色和大小被记录为太平洋蓝鳍金枪鱼的标本有T. maccoyii；而基于这些特征的新西兰水域的东方瓢虫的早期记录是不可靠的。在一些东方瓢虫标本中发现了不同寻常的颜色图案，而在麦氏瓢虫标本中没有发现。20世纪90年代，太平洋东方蓝鳍金枪鱼占新西兰专属经济区蓝鳍金枪鱼捕获量的不到0.3%。
Abstract Muscle samples were collected from 69 specimens identified as Pacific bluefin tuna (Thunnus orientalis) (Temminck and Schlegel, 1844) in the New Zealand Exclusive Economic Zone (EEZ) between 1990 and 2000. Identifications before 1996 were based on body size and colour of the caudal keel; later identifications were mostly based on the shape of abdominal cavity. The tissue samples were tested with a diagnostic mitochondrial DNA marker that distinguishes southern bluefin Thunnus maccoyii (Castelnau, 1872) and Pacific bluefin tuna T. orientalis; 59 specimens were confirmed as T. orientalis and 10 as T. maccoyii. Specimens recorded as Pacific bluefin tuna by the shape of the abdominal cavity were correctly identified as T. orientalis, and this character can be used to identify large specimens landed on tuna vessels. Some specimens recorded as Pacific bluefin tuna on the basis of colour and size were T. maccoyii; and early records of T. orientalis in New Zealand waters, based on these characters, are unreliable. Unusual colour patterns were reported in some specimens of T. orientalis but not T. maccoyii. The Pacific bluefin tuna T. orientalis accounted for less than 0.3% of the bluefin tuna catch in the New Zealand EEZ during the 1990s.