Cell-specific enhancers in the rat exocrine pancreas.
Cell-specific enhancers in the rat exocrine pancreas.
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大鼠外分泌胰腺中的细胞特异性增强剂。
DOI：
10.1073/pnas.83.11.3599
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发表时间：
1986
影响因子：
11.1
通讯作者：
Rutter,WJ
中科院分区：
文献类型：
--
作者：
Boulet,AM;Erwin,CR;Rutter,WJ
胰淀粉酶、胰凝乳蛋白酶B和胰蛋白酶I基因在胰腺外分泌中特异性表达。这些基因的5 '侧翼区指导胰腺外分泌细胞系AR 4 - 2 J中连接的报告功能（氯霉素乙酰转移酶）的优先表达。这种细胞特异性活性所需的淀粉酶和胰凝乳蛋白酶基因上游的序列具有转录增强子的特征。我们已经通过缺失分析绘制了负责增强子活性的区域。这些区域内特定核苷酸序列的修饰可以改变或消除增强子功能。比较胰腺外分泌中表达的九个基因的5 '侧翼区域，确定了一个短相关序列家族。这些序列位于增强子区域内，我们已经映射，并可能在胰腺外分泌特异性基因的表达调控中发挥作用。
Pancreatic amylase, chymotrypsin B, and trypsin I genes are specifically expressed in the exocrine pancreas. The 5'-flanking regions of these genes direct preferential expression of a linked reporter function (chloramphenicol acetyltransferase) in the pancreatic exocrine cell line AR4-2J. The sequences upstream of the amylase and chymotrypsin genes that are required for this cell specific activity possess the characteristics of transcriptional enhancers. We have mapped the regions responsible for enhancer activity by deletion analysis. Modification of specific nucleotide sequences within these regions can alter or eliminate enhancer function. Comparison of the 5'-flanking regions of nine genes expressed in the exocrine pancreas identifies a family of short related sequences. These sequences are located within the enhancer regions that we have mapped and may play a role in the regulation of the expression of pancreatic exocrine-specific genes.