Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair
Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair
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可注射海藻酸钠/PRP复合水凝胶作为软骨修复潜在细胞载体的制备和性能
DOI：
10.1002/jbm.a.36720
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发表时间：
2019-09-01
影响因子：
4.9
通讯作者：
Zhao, Mingyan
中科院分区：
文献类型：
--
作者：
Gao, Xiang;Gao, Liyang;Zhao, Mingyan
三维支架如水凝胶可用作细胞载体用于体外或体内定殖，并且已成为替代受损组织的主要研究课题。本研究以碳酸钙-D-葡萄糖酸-d-内酯（CaCO 3-GDL）为载体，制备了一种新型的海藻酸钠（SA）与富血小板血浆（PRP）复合水凝胶。结果表明，PRP的加入大大改变了复合水凝胶的物理性能和生物性能，这取决于共混比。藻酸盐水凝胶的凝胶化速率和溶胀比显着降低通过添加PRP，这也产生了更均匀的凝胶结构。场发射扫描电子显微镜（FE-SEM）研究证实了PRP衍生的蛋白质在水凝胶中的掺入，其中发现了孔径为200-300 μ m的多孔结构。与纯SA凝胶相比，SA/PRP复合水凝胶的压缩机械强度有所提高。具有最高PRP含量的复合水凝胶在0.26 MPa的最大压缩应力下表现出55%的最大应变，而纯SA水凝胶在0.08 MPa的应力下的最大压缩应变仅为45%。还发现，通过添加PRP加速了藻酸盐凝胶的体外降解。在细胞反应方面，所有凝胶均表现出优异的细胞相容性。事实上，复合水凝胶支持骨髓来源的间充质干细胞增殖及其软骨形成，并上调软骨形成标记基因Sox 9和聚集蛋白聚糖。总之，本研究表明，SA/PRP复合水凝胶作为软骨组织工程的细胞载体的巨大潜力。
Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue. In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3-GDL) was successfully prepared. It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio. The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure. Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 mu m was found. On the other hand, an increase in surface roughness was observed after the addition of PRP. The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel. The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa. It was also found that the in vitro degradation of the alginate gel was accelerated by the addition of PRP. In terms of cellular responses, all gels exhibited an excellent cytocompatibility. Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan. Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.