Acoustofluidic, Label-Free Separation and Simultaneous Concentration of Rare Tumor Cells from White Blood Cells
Acoustofluidic, Label-Free Separation and Simultaneous Concentration of Rare Tumor Cells from White Blood Cells
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DOI：
10.1021/acs.analchem.5b02023
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发表时间：
2015-09-15
影响因子：
7.4
通讯作者：
Laurell, Thomas
中科院分区：
文献类型：
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作者：
Antfolk, Maria;Magnusson, Cecilia;Laurell, Thomas
从外周血中富集稀有细胞已成为实现非侵入性诊断和开发个性化药物的手段，通常与在分子分析或培养之前浓缩富集的稀有细胞群的先决条件相关。然而，当处理低细胞数时，通过离心进行的普通浓缩具有重要的局限性。在这里，我们报告了一个集成的基于声全息的稀有细胞富集系统与集成的浓缩相结合。聚苯乙烯7克微粒可以从5 μ m颗粒中分离出来，在0.1 ± 0.03%的污染下回收率为99.3 ± 0.3%，回收的7克颗粒的总浓度为25.7 ± 1.7倍。在100 μ L/min的流速下，掺入红细胞裂解的人血中的乳腺癌细胞（MCF 7）以91.8 +/-1.0%的效率与0.6 +/-0.1%的污染从具有23.8 +/-1.3倍癌细胞浓度的白色血细胞中分离。加标至全血中的前列腺癌细胞（DU 145）的回收率为84.1 +/- 2.1%，白色血细胞污染率为0.2 +/- 0.04%，癌细胞浓度为9.6 +/- 0.4倍。这种同时在芯片上的分离和浓缩显示了未来声流体系统在临床实践中使用外周静脉血进行循环肿瘤细胞的快速无标记富集和分子表征的可行性。
Enrichment of rare cells from peripheral blood has emerged as a means to enable noninvasive diagnostics and development of personalized drugs, commonly associated with a prerequisite to concentrate the enriched rare cell population prior to molecular analysis or culture. However, common concentration by centrifugation has important limitations when processing low cell numbers. Here, we report on an integrated acoustophoresis-based rare cell enrichment system combined with integrated concentration. Polystyrene 7 gm microparticles could be separated from 5,mu m particles with a recovery of 99.3 +/- 0.3% at a contamination of 0.1 +/- 0.03%, with an overall 25.7 +/- 1.7-fold concentration of the recovered 7 gm particles. At a flow rate of 100 mu L/min, breast cancer cells (MCF7) spiked into red blood cell-lysed human blood were separated with an efficiency of 91.8 +/- 1.0% with a contamination of 0.6 +/- 0.1% from white blood cells with a 23.8 +/- 1.3-fold concentration of cancer cells. The recovery of prostate cancer cells (DU145) spiked into whole blood was 84.1 +/- 2.1% with 0.2 +/- 0.04% contamination of white blood cells with a 9.6 +/- 0.4-fold concentration of cancer cells. This simultaneous on-chip separation and concentration shows feasibility of future acoustofluidic systems for rapid label-free enrichment and molecular characterization of circulating tumor cells using peripheral venous blood in clinical practice.