The total number of neurons and calcium binding protein positive neurons during aging in the cochlear nucleus of CBA/CaJ mice: a quantitative study
The total number of neurons and calcium binding protein positive neurons during aging in the cochlear nucleus of CBA/CaJ mice: a quantitative study
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DOI：
10.1016/s0378-5955(01)00295-7
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发表时间：
2001-08-01
期刊：
影响因子：
2.8
通讯作者：
Bogdanovic, N
中科院分区：
文献类型：
--
作者：
Idrizbegovic, E;Canlon, B;Bogdanovic, N
采用定量体视学方法，光学分级仪，测定了CBA/CaJ（CBA）小鼠在衰老过程中（1-39月龄）耳蜗背侧核和后腹侧核（DCN和PVCN）中神经元总数和用小清蛋白、钙结合蛋白-D28 k（钙结合蛋白）和钙视网膜蛋白免疫染色的神经元总数。CBA小鼠在生命后期仅具有适度的感觉神经病理学。DCN神经元总数随年龄增长而减少（r =-0.54，P < 0.03），而PVCN神经元总数与年龄无明显差异（r = 0.16，P = 0.57）。在DCN 5.5%的神经元是小白蛋白阳性的非常老（30 - 39个月）的小鼠，与2.2%的1个月大的小鼠。在DCN中，30-39月龄小鼠中3%的神经元为钙结合蛋白免疫阳性，而1月龄组为1.9%。在PVCN中。在非常老的小鼠中，20%的神经元是小清蛋白免疫阳性的。相比之下，年轻的老鼠只有12%。钙结合蛋白在PVCN中未显示任何显著的年龄相关差异。在DCN和PVCN的calretinin免疫阳性神经元的总数没有显示出任何显着的变化，随着年龄的增长。总之。DCN和PVCN中的神经元总数与年龄相关且具有区域特异性。而在DCN和PVCN的神经元数量减少或不变，分别，钙结合蛋白阳性神经元的数量呈梯度增加，在区域特异性和蛋白特异性的方式在老化过程中。(C)出版社：Elsevier Science B. V.
The quantitative stereological method, the optical fractionator, was used for determining the total number of neurons and the total number of neurons immunostained with parvalbumin, calbindin-D28k (calbindin), and calretinin in the dorsal and posteroventral cochlear nucleus (DCN and PVCN) in CBA/CaJ (CBA) mice during aging (1-39 months old). CBA mice have only a modest sensorineural pathology late in life. An age-related decrease of the total number of neurons was demonstrated in the DCN (r = -0.54, P < 0.03), while the total number of neurons in the PVCN did not show any significant age-related differences (r = 0.16, P = 0.57). In the DCN 5.5% of neurons were parvalbumin positive in the very old (30 39 months) mice, vs. 2.2% in the 1 month old mice. In the DCN 3% of the neurons were calbindin immunopositive in the 30-39 months mice compared to 1.9% in the I month old group. In the PVCN. 20% of the neurons in the very old mice were parvalbumin immunopositive. compared to 12% in the young mice. Calbindin did not show any significant age-related differences in the PVCN. The total number of calretinin immunopositive neurons both in the DCN and PVCN did not show any significant change with increasing age. In conclusion. the total neuronal number in the DCN and PVCN was age-related and region-specific. While the neuronal number in the DCN and PVCN was decreased or unchanged, respectively, the calcium binding protein positive neuronal number showed a graded increase during aging in a region-specific and protein-specific manner. (C) 2001 Published by Elsevier Science B.V.