Effect of temperature on the photoproperties of luminescent terbium sensors for homogeneous bioassays.
Effect of temperature on the photoproperties of luminescent terbium sensors for homogeneous bioassays.
复制标题
DOI：
10.1002/bio.2355
复制
发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Z. Dong;Yingsong Wu;Zheng Wang;An He;Ming Li;Mei-Jun Chen;Hong-yan Du;Qiang Ma;Tiancai Liu
中科院分区：
文献类型：
--
作者：
Z. Dong;Yingsong Wu;Zheng Wang;An He;Ming Li;Mei-Jun Chen;Hong-yan Du;Qiang Ma;Tiancai Liu
我们开发了一种基于能量共振转移的发光铽传感器（LTS），用于均质生物检测。研究了温度对LTS光致发光和时间分辨荧光的影响。当温度从277 K升高到369 K时，光致发光量子产率下降了25%，时间分辨荧光下降了54%，寿命明显缩短。研究表明，将样品从298加热到310、333或369 K，然后冷却到298 K后，光致发光和时间分辨荧光量子产率都有很大程度的恢复。这些结果表明，LTS均相生物测定对温度敏感，应在恒定温度下进行，以确保温度效应不影响数据，提高结果的准确性。本研究结果对LTS在均相生物测定中的应用具有重要意义。
We developed a luminescent terbium sensor (LTS) based on energy resonance transfer for homogeneous bioassays. The effect of temperature on photoluminescence and time-resolved fluorescence of the LTS was investigated. When the temperature was increased from 277 K to 369 K, the photoluminescence quantum yield decreased by up to 25 %, time-resolved fluorescence decreased by up to 54 %, and the lifetime shortened dramatically. Studies showed that both photoluminescence and time-resolved fluorescence quantum yields were largely recovered after samples were heated from 298 to 310, 333 or 369 K and subsequently cooled to 298 K. These results indicate that the homogeneous bioassay with LTS is sensitive to temperature and should be conducted at a constant temperature to ensure the temperature effect does not influence data and to increase the accuracy of the results. The results of this study are important for LTS applications in homogeneous bioassays.