Polyamine regulates tolerance to water stress in leaves of white clover associated with antioxidant defense and dehydrin genes via involvement in calcium messenger system and hydrogen peroxide signaling.
Polyamine regulates tolerance to water stress in leaves of white clover associated with antioxidant defense and dehydrin genes via involvement in calcium messenger system and hydrogen peroxide signaling.
复制标题
多胺通过参与钙信使系统和过氧化氢信号调节与抗氧化防御和脱水基因相关的白三叶草叶片对水分胁迫的耐受性
DOI：
10.3389/fphys.2015.00280
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发表时间：
2015
影响因子：
4
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Li Z;Zhang Y;Peng D;Wang X;Peng Y;He X;Zhang X;Ma X;Huang L;Yan Y
内源多胺（PA）作为一种信号分子激活剂，在植物对水分胁迫的耐受中起着重要作用。水分胁迫导致叶片内源腐胺（Put）、亚精胺（Spd）和精胺（Spm）含量增加。外源Spd可诱导H2 O2瞬间爆发和胞浆游离Ca ~（2+）积累，并激活NADPH氧化酶和CDPK基因表达。PA生物合成抑制剂在很大程度上降低了水分胁迫诱导的H2 O2积累、胞内游离Ca 2+释放、抗氧化酶活性和基因表达，加重了水分胁迫诱导的氧化损伤，而外源Spd的加入则减轻了这些抑制作用，表明PA参与了水分胁迫诱导的H2 O2和胞内游离Ca 2+的产生以及胁迫耐受。脱氢酶基因（Y2 SK、Y2K和SK 2）对外源Spd具有高度响应性。H_2O_2清除剂、H_2O_2生成抑制剂、钙通道阻断剂、钙调素拮抗剂和CDPK抑制剂均能阻断PA诱导的抗氧化防御和抗氧化蛋白基因表达。这些结果表明，PA调节耐受性的水分胁迫白色三叶草与抗氧化防御和甜菜碱通过参与钙信使系统和H2 O2信号通路。PA诱导的H2 O2产生需要Ca 2+释放，而PA诱导的Ca 2+释放对H2 O2产生也是必不可少的，表明PA诱导的H2 O2和Ca 2+信号之间存在相互作用。
Endogenous polyamine (PA) may play a critical role in tolerance to water stress in plants acting as a signaling molecule activator. Water stress caused increases in endogenous PA content in leaves, including putrescine (Put), spermidine (Spd), and spermine (Spm). Exogenous application of Spd could induce the instantaneous H2O2 burst and accumulation of cytosolic free Ca2+, and activate NADPH oxidase and CDPK gene expression in cells. To a great extent, PA biosynthetic inhibitor reduced the water stress-induced H2O2 accumulation, free cytosolic Ca2+ release, antioxidant enzyme activities and genes expression leading to aggravate water stress-induced oxidative damage, while these suppressing effects were alleviated by the addition of exogenous Spd, indicating PA was involved in water stress-induced H2O2 and cytosolic free Ca2+ production as well as stress tolerance. Dehydrin genes (Y2SK, Y2K, and SK2) were showed to be highly responsive to exogenous Spd. PA-induced antioxidant defense and dehydrin genes expression could be blocked by the scavenger of H2O2 and the inhibitors of H2O2 generation or Ca2+ channels blockers, a calmodulin antagonist, as well as the inhibitor of CDPK. These findings suggested that PA regulated tolerance to water stress in white clover associated with antioxidant defenses and dehydrins via involvement in the calcium messenger system and H2O2 signaling pathways. PA-induced H2O2 production required Ca2+ release, while PA-induced Ca2+ release was also essential for H2O2 production, suggesting an interaction between PA-induced H2O2 and Ca2+ signaling.