A minor outer capsid protein, P9, of Rice dwarf virus
A minor outer capsid protein, P9, of Rice dwarf virus
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DOI：
10.1007/s00705-003-0160-3
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发表时间：
2003-11
影响因子：
2.7
通讯作者：
B. Zhong;A. Kikuchi;Y. Moriyasu;T. Higashi;K. Hagiwara;T. Omura
中科院分区：
文献类型：
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作者：
B. Zhong;A. Kikuchi;Y. Moriyasu;T. Higashi;K. Hagiwara;T. Omura
纯化的水稻矮缩病毒（RDV）中有一个30 kDa的小分子多肽，其部分氨基酸序列与该病毒dsRNA片段S9编码的推测氨基酸序列一致。该多肽通过免疫印迹分析特异性检测，该分析使用针对杆状病毒系统中表达的S9产物产生的抗体。用相对较高浓度的MgCl 2处理纯化的RDV颗粒，将多肽与其他外壳蛋白一起从核心颗粒中除去。这些结果表明，30 kDa多肽是由RDV的基因组区段S9编码的次要外壳蛋白。这种蛋白质被命名为P9蛋白。
Partial amino acid sequence of a minor 30 kDa polypeptide in purifiedRice dwarf virus(RDV) was identical to the deduced amino acid sequence encoded by the dsRNA segment S9 of the virus. This polypeptide was specifically detected by Western blotting analysis with antibodies raised against the product of S9 expressed in a baculovirus system. Treatment of purified RDV particles with a relatively higher concentration of MgCl2removed the polypeptide from core particles together with other outer capsid proteins. These results demonstrate that the 30 kDa polypeptide is a minor outer capsid protein that is encoded by genome segment S9 of RDV. This protein was named P9 protein.