Calcium dynamics of spines depend on their dendritic location
Calcium dynamics of spines depend on their dendritic location
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DOI：
10.1016/s0896-6273(02)00576-7
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发表时间：
2002-01-31
期刊：
影响因子：
16.2
通讯作者：
Yuste, R
中科院分区：
文献类型：
--
作者：
Holthoff, K;Tsay, D;Yuste, R
树突棘在形态和功能上是异质的。为了理解这种多样性，我们使用第5层新皮质锥体细胞的双光子成像，并测量动作电位诱发的[Ca2+]（i）在脊柱中的瞬变。脊柱钙动力学由（i）母体树突的直径、（ii）脊柱颈的长度和（iii）脊柱钙泵的强度控制。这些因素产生不同的钙动力学的棘从基底，近端顶端，和远端顶端树突，差异更明显，没有外源性缓冲液。在近端和远端的顶端树突，不同的钙动力学与不同的易感性突触抑郁症，和修改钙动力学的棘改变长期抑郁症的表达。因此，脊柱的位置显然决定其钙动力学和突触可塑性。我们的研究结果突出了新皮层神经元设计的精确性。
Dendritic spines are morphologically and functionally heterogeneous. To understand this diversity, we use two-photon imaging of layer 5 neocortical pyramidal cells and measure action potential-evoked [Ca2+](i) transients in spines. Spine calcium kinetics are controlled by (i) the diameter of the parent dendrite, (ii) the length of the spine neck, and (iii) the strength of spine calcium pumps. These factors produce different calcium dynamics in spines from basal, proximal apical, and distal apical dendrites, differences that are more pronounced without exogenous buffers. In proximal and distal apical dendrites, different calcium dynamics correlate with different susceptibility to synaptic depression, and modifying calcium kinetics in spines changes the expression of long-term depression. Thus, the spine location apparently determines its calcium dynamics and synaptic plasticity. Our results highlight the precision in design of neocortical neurons.