New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control
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DOI：
10.1073/pnas.0308014101
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发表时间：
2004-04-27
影响因子：
11.1
通讯作者：
Breaker, RR
中科院分区：
文献类型：
--
作者：
Barrick, JE;Corbino, KA;Breaker, RR
参与基础代谢的某些基因的表达受到嵌入其相应 mRNA 中的代谢物结合“核糖开关”元件的调节。我们在枯草芽孢杆菌基因组中鉴定出至少六个具有核糖开关功能特征的元件（glmS、gcvT、ydaO/yuaA、ykkC/yxkD、ykoK 和 yybP/ykoY）。这些基序在许多细菌物种中表现出广泛的序列和二级结构保守性，并且出现在相关基因的上游。在革兰氏阳性生物体中，位于 glmS 基因上游的元件充当代谢物依赖性核酶，对 6-磷酸葡萄糖胺做出反应。其他基序在转录为 RNA 分子时形成复杂的折叠结构并带有内在的终止子结构。这些发现表明核糖开关是许多微生物物种中控制代谢基因的主要遗传调控机制。
The expression of certain genes involved in fundamental metabolism is regulated by metabolite-binding "riboswitch" elements embedded within their corresponding mRNAs. We have identified at least six additional elements within the Bacillus subtilis genome that exhibit characteristics of riboswitch function (glmS, gcvT, ydaO/yuaA, ykkC/yxkD, ykoK, and yybP/ykoY). These motifs exhibit extensive sequence and secondary-structure conservation among many bacterial species and occur upstream of related genes. The element located upstream of the glmS gene in Gram-positive organisms functions as a metabolite-dependent ribozyme that responds to glucosamine-6-phosphate. Other motifs form complex folded structures when transcribed as RNA molecules and carry intrinsic terminator structures. These findings indicate that riboswitches serve as a major genetic regulatory mechanism for the control of metabolic genes in many microbial species.