An in vitro traumatic injury model to examine the response of neurons to a hydrodynamically-induced deformation
An in vitro traumatic injury model to examine the response of neurons to a hydrodynamically-induced deformation
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DOI：
10.1007/bf02684844
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发表时间：
1997-07-01
影响因子：
3.8
通讯作者：
Thibault, LE
中科院分区：
文献类型：
--
作者：
LaPlaca, MC;Thibault, LE
开发了一种新的体外系统来研究创伤性机械负荷对单个细胞的影响。细胞剪切损伤装置（CSID）是一种平行圆盘粘度计，其施加具有可变起始速率的流体剪切应力。CSID与显微镜和生物化学技术结合使用，以获得神经元对损伤的变形和功能反应的定量表达。分析和数值近似的剪应力在底盘进行了比较，以确定二次流的贡献。在启动过程中，剪切应力的很大一部分指向r方向，因此完整的Navier-Stokes方程对于准确描述瞬态剪切应力是必要的。当以高速率（800达因cm（-2）sec（-1））对培养的神经元施加剪切应力时，获得了一系列细胞膜应变（0.01至0.53），表明细胞反应的不均匀性。在功能上，细胞溶质钙和细胞外乳酸脱氢酶水平增加响应高应变率（>1秒（-1））负荷，与准静态（
A novel in vitro system was developed to examine the effects of traumatic mechanical loading on individual cells. The cell shearing injury device (CSID) is a parallel disk viscometer that applies fluid shear stress with variable onset rate. The CSID was used in conjunction with microscopy and biochemical techniques to obtain a quantitative expression of the deformation and functional response of neurons to injury. Analytical and numerical approximations of the shear stress at the bottom disk were compared to determine the contribution of secondary flows. A significant portion of the shear stress was directed in the r-direction during start-up, and therefore the full Navier-Stokes equation was necessary to accurately describe the transient shear stress. When shear stress was applied at a high rate (800 dyne cm(-2) sec(-1)) to cultured neurons, a range of cell membrane strains (0.01 to 0.53) was obtained, suggesting inhomogeneity in cellular response. Functionally, cytosolic calcium and extracellular lactate dehydrogenase levels increased in response to high strain rate (>1 sec(-1)) loading, compared with quasistatic (