Beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold.
Beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold.
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DOI：
10.3389/fmicb.2015.00755
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发表时间：
2015
影响因子：
5.2
通讯作者：
Scott JK
中科院分区：
文献类型：
--
作者：
Henry KA;Arbabi-Ghahroudi M;Scott JK
在过去的25年里，噬菌体展示技术已经成为研究蛋白质相互作用的宝贵工具。然而，丝状噬菌体固有的生物学、生物化学和生物物理特性，以及其基因操作的便捷性，也使其成为传统噬菌体展示经典之外的一个有吸引力的平台。本文将重点介绍丝状噬菌体的独特性质及其在当前研究中的广泛应用。特别强调的是：（一）噬菌体作为疫苗载体的优势，包括其高免疫原性、相对简单的抗原性和激活一系列免疫反应的能力；（二）噬菌体作为传染病和慢性疾病的预防和治疗剂的潜力；（三）病毒粒子主要外壳蛋白晶格的规律性，使其能够进行各种生物偶联和表面化学应用，特别是在纳米材料中；（iv）噬菌体的大群体规模和快速世代，使其成为定向蛋白质进化的优秀模型系统。尽管它们在生物圈中无处不在，但宏基因组学工作刚刚开始探索丝状和非丝状噬菌体的生态学，以及它们在细菌种群进化中的作用。因此，丝状噬菌体是一种健壮、廉价、多功能的微生物，其生物工程应用不断向新的方向扩展，尽管它在某些领域的局限性阻碍了它的广泛采用和使用。
For the past 25 years, phage display technology has been an invaluable tool for studies of protein–protein interactions. However, the inherent biological, biochemical, and biophysical properties of filamentous bacteriophage, as well as the ease of its genetic manipulation, also make it an attractive platform outside the traditional phage display canon. This review will focus on the unique properties of the filamentous bacteriophage and highlight its diverse applications in current research. Particular emphases are placed on: (i) the advantages of the phage as a vaccine carrier, including its high immunogenicity, relative antigenic simplicity and ability to activate a range of immune responses, (ii) the phage’s potential as a prophylactic and therapeutic agent for infectious and chronic diseases, (iii) the regularity of the virion major coat protein lattice, which enables a variety of bioconjugation and surface chemistry applications, particularly in nanomaterials, and (iv) the phage’s large population sizes and fast generation times, which make it an excellent model system for directed protein evolution. Despite their ubiquity in the biosphere, metagenomics work is just beginning to explore the ecology of filamentous and non-filamentous phage, and their role in the evolution of bacterial populations. Thus, the filamentous phage represents a robust, inexpensive, and versatile microorganism whose bioengineering applications continue to expand in new directions, although its limitations in some spheres impose obstacles to its widespread adoption and use.