XX/XY Sex Chromosomes in the South American Dwarf Gecko (Gonatodes humeralis)
XX/XY Sex Chromosomes in the South American Dwarf Gecko (Gonatodes humeralis)
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DOI：
10.1093/jhered/esx112
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发表时间：
2018-06-01
影响因子：
3.1
通讯作者：
Higham, Timothy E.
中科院分区：
文献类型：
--
作者：
Gamble, Tony;McKenna, Erin;Higham, Timothy E.
使用限制性位点相关DNA测序（RADseq）鉴定的性别特异性遗传标记允许在标准细胞遗传学方法失败的情况下识别物种的性染色体系统。因此，具有雄性特异性RAD标记的物种具有XX/XY性染色体系统（雄性异配性），而具有雌性特异性RAD标记的物种具有ZZ/ZW性染色体（雌性异配性）。在这里，我们使用来自5只雄性和5只雌性南美侏儒壁虎（Gonatodes humeralis）的RADseq数据来识别XX/XY性染色体系统。这是第一个确信已知的性染色体系统在Gonatodes物种。我们使用了低覆盖率的从头G。humeralis基因组组装来设计PCR引物，以验证性别特异性RADseq标记的子集的雄性特异性，并描述即使是适度的基因组组装也可以促进具有不同性染色体系统的物种中性别特异性PCR引物的设计。
Sex-specific genetic markers identified using restriction site-associated DNA sequencing, or RADseq, permits the recognition of a species' sex chromosome system in cases where standard cytogenetic methods fail. Thus, species with male-specific RAD markers have an XX/XY sex chromosome system (male heterogamety) while species with female-specific RAD markers have a ZZ/ZW sex chromosome (female heterogamety). Here, we use RADseq data from 5 male and 5 female South American dwarf geckos (Gonatodes humeralis) to identify an XX/XY sex chromosome system. This is the first confidently known sex chromosome system in a Gonatodes species. We used a low-coverage de novo G. humeralis genome assembly to design PCR primers to validate the male-specificity of a subset of the sex-specific RADseq markers and describe how even modest genome assemblies can facilitate the design of sex-specific PCR primers in species with diverse sex chromosome systems.