Topographic Mapping between Basal Forebrain Cholinergic Neurons and the Medial Prefrontal Cortex in Mice
Topographic Mapping between Basal Forebrain Cholinergic Neurons and the Medial Prefrontal Cortex in Mice
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DOI：
10.1523/jneurosci.3011-14.2014
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发表时间：
2014-12-03
影响因子：
5.3
通讯作者：
Wouterlood, Floris G.
中科院分区：
文献类型：
--
作者：
Bloem, Bernard;Schoppink, Luc;Wouterlood, Floris G.
基底前脑内侧前额叶皮质(MPFC)的胆碱能神经支配对认知功能起着至关重要的作用。然而，人们对小鼠基底前脑和mPFC之间的连接组织知之甚少。通过局灶性病毒注射诱导ChAT-IRES-CRE小鼠依赖Cre增强的黄色荧光蛋白表达，我们检验了基底前脑胆碱能神经元与它们向mPFC的轴突投射之间存在地形图的假设。我们发现，上升至PFC的胆碱能纤维遵循四条路径，胆碱能神经元根据它们在基底前脑中的位置而选择这些路径。此外，还观察到了一个一般的标测模式，即在基底前脑内，胆碱能神经元沿嘴到尾的位置与mPFC内胆碱能纤维分布的腹向向背和嘴向尾的移位相关。最后，我们发现基底前脑吻侧部和尾侧部的神经元对mPFC腹侧的浅层和深层有不同的神经支配。因此，存在胆碱能系统的额尾部组织，其中根据胆碱能神经元在基底前脑中的位置，以不同的mPFC区域和皮质层为靶点。
The basal forebrain cholinergic innervation of the medial prefrontal cortex (mPFC) is crucial for cognitive performance. However, little is known about the organization of connectivity between the basal forebrain and the mPFC in the mouse. Using focal virus injections inducing Cre-dependent enhanced yellow fluorescent protein expression in ChAT-IRES-Cre mice, we tested the hypothesis that there is a topographic mapping between the basal forebrain cholinergic neurons and their axonal projections to the mPFC. We found that ascending cholinergic fibers to them PFC follow four pathways and that cholinergic neurons take these routes depending on their location in the basal forebrain. In addition, a general mapping pattern was observed in which the position of cholinergic neurons measured along a rostral to caudal extent in the basal forebrain correlated with a ventral to dorsal and a rostral to caudal shift of cholinergic fiber distribution in mPFC. Finally, we found that neurons in the rostral and caudal parts of the basal forebrain differentially innervate the superficial and deep layers of the ventral regions of the mPFC. Thus, a frontocaudal organization of the cholinergic system exists in which distinct mPFC areas and cortical layers are targeted depending on the location of the cholinergic neuron in the basal forebrain.