The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cells
The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cells
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DOI：
10.1128/mcb.24.10.4207-4220.2004
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发表时间：
2004-05-01
影响因子：
5.3
通讯作者：
Okuda, A
中科院分区：
文献类型：
--
作者：
Miyagi, S;Saito, T;Okuda, A
Sox-2基因在胚胎干细胞和神经干细胞中表达。两个转录增强子区域，Sox-2调节区1（SRR 1）和SRR 2，先前描述了基于它们在ES细胞中的活性。在这里，我们证明了这些调控区域也发挥其在神经干细胞中的活动。此外，我们的数据显示，在ES细胞中，SRR 1和SRR 2显示其活动，而特别是在多能神经干细胞或祖细胞，但停止在分化的细胞，如有丝分裂后的神经元的功能。系统的缺失和突变分析表明，SRR 2的相同或至少重叠的DNA元件参与其在ES和神经干细胞或祖细胞中的活性。因此，SRR 2是增强子的第一个例子，其中单个调控核心序列参与两种不同干细胞中的多能状态特异性表达，即，胚胎干细胞和神经干细胞。
The Sox-2 gene is expressed in embryonic stem (ES) cells and neural stem cells. Two transcription enhancer regions, Sox-2 regulatory region 1 (SRR1) and SRR2, were described previously based on their activities in ES cells. Here, we demonstrate that these regulatory regions also exert their activities in neural stem cells. Moreover, our data reveal that, as in ES cells, both SRR1 and SRR2 show their activities rather specifically in multipotent neural stem or progenitor cells but cease to function in differentiated cells, such as postmitotic neurons. Systematic deletion and mutation analyses showed that the same or at least overlapping DNA elements of SRR2 are involved in its activity in both ES and neural stem or progenitor cells. Thus, SRR2 is the first example of an enhancer in which a single regulatory core sequence is involved in multipotent-state-specific expression in two different stem cells, i.e., ES and neural stem cells.