EFFECT OF FOOD MANIPULATION ON THE GNRH-LH-ESTRADIOL AXIS OF YOUNG FEMALE RATS
EFFECT OF FOOD MANIPULATION ON THE GNRH-LH-ESTRADIOL AXIS OF YOUNG FEMALE RATS
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DOI：
10.1152/ajpregu.1988.254.4.r616
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发表时间：
1988-04-01
影响因子：
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通讯作者：
BRONSON, FH
中科院分区：
文献类型：
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作者：
BRONSON, FH
通过限制雌性大鼠从80-85g(27-29日龄)到2个月龄的食物摄入量，防止雌性大鼠的生长发育。恢复自由喂养通常会在第三或第四个暗期产生青春期排卵。自由采食48h可增加去卵巢雌性动物黄体生成素(L H)的脉动频率。这种治疗还降低了植入硅胶胶囊的卵巢切除女性的循环雌二醇水平。但对1h血雌二醇清除率无影响，也不影响下丘脑可测促性腺激素释放激素(GnRH)池、脑垂体可测促性腺激素池、垂体腺对GnRH的反应或血中促黄体生成素清除率。综上所述，目前的结果表明，食物限制通过卵巢类固醇非依赖性途径对GnRH的分泌起相当特异的作用，从而抑制青春期发育。对于增强的负反馈敏感性的假定补充作用不能被评估，因为包埋的雌二醇的异常结果。
Female rats were prevented from growing and developing reproductively by restricting their food intake from the time they reached 80-85 g (27-29 days of age) until they were 2 mo old. A return to ad libitum feeding then typically yielded the pubertal ovulation during the third or fourth dark period. Ad libitum feeding for 48 h increased the frequency of luteinizing hormone (LH) pulsing in ovariectomized females. This treatment also depressed the level of circulating estradiol in ovariectomized females implanted with Silastic capsules. It had no effect on the rate at which estradiol was cleared from the blood in a 1-h test, however, nor did it affect the pool of assayable gonadotropic-releasing hormone (GnRH) in the hypothalamus, the pool of assayable LH in the pituitary, the response of the pituitary to GnRH, or the rate at which LH was cleared from the blood. In toto, the present results suggest that food restriction inhibits pubertal development by acting rather specifically on GnRH secretion via an ovarian steroid-independent pathway. The presumed supplemental role for enhanced negative-feedback sensitivity could not be evaluated because of the aberrant results with encapsulated estradiol.