Use of an animal model for the study of the role of human immunodeficiency virus 1 in the human intestine.
Use of an animal model for the study of the role of human immunodeficiency virus 1 in the human intestine.
复制标题
使用动物模型研究人类免疫缺陷病毒 1 在人类肠道中的作用。
DOI：
10.1016/0016-5085(92)90166-v
复制
发表时间：
1992
期刊：
影响因子：
29.4
通讯作者：
Letvin,NL
中科院分区：
文献类型：
--
作者：
Winter,HS;Fox,CH;Hendren,RB;Isselbacher,KJ;Folkman,J;Letvin,NL
人类免疫缺陷病毒 (HIV)-1 疾病的胃肠道表现集中于肠道功能障碍的可识别原因，例如寄生虫和细菌病原体。肠道固有层含有携带 HIV-1 的细胞，但这一观察结果的意义仍不清楚。由于可用于评估这种感染的胃肠道影响的动物模型有限，因此开发了一种将人胎儿肠皮下移植到免疫缺陷小鼠背部的系统。 8周后，胎儿组织成熟为具有管腔的成人组织。将无细胞的 HIV-1 接种到外植体中，并通过组织学评估、原位杂交和聚合酶链反应来评估感染证据。没有发现 HIV-1 掺入上皮细胞的证据。结论是，该模型提供了一个可以评估肠道 HIV-1 相互作用的系统。在此模型中，无细胞 HIV-1 似乎不会感染上皮细胞。
The gastrointestinal manifestations of human immunodeficiency virus (HIV)-1 disease have centered on identifiable causes of intestinal dysfunction such as parasitic and bacterial pathogens. The lamina propria of the intestine contains cells that harbor HIV-1, but the significance of this observation remains unknown. Because limited animal models are available to evaluate the gastrointestinal effects of this infection, a system that uses human fetal intestine transplanted subcutaneously onto the back of an immunodeficient mouse was developed. After 8 weeks, fetal tissues mature into an adult-appearing tissue with a lumen. Cell-free HIV-1 was inoculated into the expiants, and evidence for infection was evaluated by histological evaluation, in situ hybridization, and polymerase chain reaction. No evidence for HIV-1 incorporation into epithelial cells could be found. It was concluded that this model provides a system in which intestinal HIV-1 interaction can be evaluated. In this model, cell-free HIV-1 does not appear to infect the epithelial cell.