Novel Tat-encoding bicistronic human immunodeficiency virus type 1-based gene transfer vectors for high-level transgene expression.
Novel Tat-encoding bicistronic human immunodeficiency virus type 1-based gene transfer vectors for high-level transgene expression.
复制标题
新型 Tat 编码双顺反子人类免疫缺陷病毒 1 型基因转移载体，用于高水平转基因表达。
DOI：
10.1128/jvi.74.14.6659-6668.2000
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发表时间：
2000
影响因子：
5.4
通讯作者：
Schuening,F
中科院分区：
文献类型：
--
作者：
Srinivasakumar,N;Schuening,F
我们描述了基于人类免疫缺陷病毒1型（HIV-1）的双顺反子单外显子达特（72-氨基酸达特[Tat 72]）和全长达特（Tat 86）编码基因转移载体。我们通过使用Mason-Pfizer猴病毒（MPMV）的组成型转运元件（CTE）创建了这些载体的版本，使其不依赖于Rev。在基因转移实验中，编码Tat 72的载体比表达Tat 86的载体表现更好。在Rev独立包装系统中生产的含CTE载体的基因转移效率几乎等同于使用基于组合RNA转运（CTE和Rev-Rev响应元件）的包装系统生产的基因转移效率。Tat 72编码载体可以有效地转导到各种细胞类型中，显示出比具有猿猴巨细胞病毒立即早期或猿猴病毒40早期启动子的载体更高的转基因表达水平，并提供了具有内部启动子的HIV-1载体的替代方案。
We describe bicistronic single-exon Tat (72-amino-acid Tat [Tat72])- and full-length Tat (Tat86)-encoding gene transfer vectors based on human immunodeficiency virus type 1 (HIV-1). We created versions of these vectors that were rendered Rev independent by using the constitutive transport element (CTE) from Mason-Pfizer monkey virus (MPMV). Tat72-encoding vectors performed better than Tat86-expressing vectors in gene transfer experiments. CTE-containing vectors, produced in a Rev-independent packaging system, had gene transfer efficiencies nearly equivalent to those produced using a combination RNA transport (CTE and Rev-Rev response element)-based packaging system. The Tat72-encoding vectors could be efficiently transduced into a variety of cell types, showed higher levels of transgene expression than vectors with the simian cytomegalovirus immediate-early or the simian virus 40 early promoter, and provide an alternative to HIV-1 vectors with internal promoters.