CALCITONIN GENE-RELATED PEPTIDE (CGRP) IN CAPSAICIN-SENSITIVE SUBSTANCE-P-IMMUNOREACTIVE SENSORY NEURONS IN ANIMALS AND MAN - DISTRIBUTION AND RELEASE BY CAPSAICIN
CALCITONIN GENE-RELATED PEPTIDE (CGRP) IN CAPSAICIN-SENSITIVE SUBSTANCE-P-IMMUNOREACTIVE SENSORY NEURONS IN ANIMALS AND MAN - DISTRIBUTION AND RELEASE BY CAPSAICIN
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DOI：
10.1016/0196-9781(87)90117-3
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发表时间：
1987-03-01
期刊：
影响因子：
3
通讯作者：
FISCHER, JA
中科院分区：
文献类型：
--
作者：
FRANCOCERECEDA, A;HENKE, H;FISCHER, JA
用免疫组织化学和放射免疫分析结合高效液相色谱法（HPLC）观察了大鼠感觉神经元内降钙素基因相关肽（CGRP）样免疫反应性（-LI）的表达。在豚鼠和人的心脏、输尿管、子宫和胆囊等外周器官中均存在CGRP免疫反应（-IR）神经纤维，脊髓背角内CGRP免疫反应神经、胸脊神经节和结状神经节内阳性胞体及外周器官内神经的分布与P物质-LI密切相关。双染实验表明，在大多数情况下，周围CGRP-IR神经末梢也含有SP-LI。但在孤束核和脊髓前角的SP和CGRP免疫阳性神经元的分布不同。在心脏中，CGRP-IR神经与心肌细胞（主要是心房）、冠状血管、局部副交感神经节以及心外膜和心内膜相关。在心房中观察到的天然CGRP-LI水平比心室中高3至4倍。HPLC分析显示，大鼠和人心脏中CGRP-LI的主峰与合成的等价物具有相同的保留时间。全身辣椒素预处理的成年豚鼠引起的损失CGRP-IR终端在脊髓背角以及在外周器官，包括心脏。辣椒素处理后，CGRP-IR的含量在心脏中减少了70%，在脊髓背侧部分减少了60%。在用来自大鼠脊髓的切片进行的灌流实验中，60 mM K+和3 μ M辣椒素以钙依赖性方式诱导CGRP-LI的释放。
The presence of calcitonin-gene related peptide (CGRP)-like immunoreactivity (-LI) in sensory neurons was established by immunohistochemistry and radioimmunoassay (RIA) in combination with higher performance liquid chromatography (HPLC). CGRP-immunoreactive (-IR) nerve fibres were present in many peripheral organs including heart, ureter, uterus and gall bladder of guinea-pig and man. The distribution of CGRP-IR nerves in the dorsal horn of the spinal cord, of positive cell bodies in thoracic spinal and nodose ganglia and nerves in peripheral organs was closely related to that of substance P-LI. Double staining experiments revealed that in most cases peripheral CGRP-IR nerve terminals also contained SP-LI. However, different localization of SP and CGRP-IR neurons was observed in the nucleus of the solitary tract as well as in the ventral horn of the spinal cord. In the heart, CGRP-IR nerves were associated with myocardial cells (mainly atria), coronary vessels, local parasympathetic ganglia as well as with the epi- and endocardia. Three to 4-fold higher levels of native CGRP-LI were observed in the atria than in the ventricles of the heart. HPLC analysis revealed that the major peak of CGRP-LI in the heart of rat and man had the same retention times as the synthetic equivalents. Systemic capsaicin pretreatment of adult guinea-pigs caused a loss of CGRP-IR terminals in the dorsal horn of the spinal cord as well as in peripheral organs including the heart. After capsaicin treatment, the content of CGRP-IR was reduced by 70% in the heart and by 60% in the dorsal part of the spinal cord. In superfusion experiments with slices from the rat spinal cord, a release of CGRP-LI was induced by 60 mM K+ and 3 .mu.M capsaicin in a calcium-dependent manner.