Biosynthesis of innovative calcium phosphate/hydrogel composites: physicochemical and biological characterisation
Biosynthesis of innovative calcium phosphate/hydrogel composites: physicochemical and biological characterisation
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DOI：
10.1088/1361-6528/abc5f6
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发表时间：
2021-02-26
期刊：
影响因子：
3.5
通讯作者：
Palocci, Cleofe
中科院分区：
文献类型：
--
作者：
Chronopoulou, Laura;Cacciotti, Ilaria;Palocci, Cleofe
支持和引导组织再生的目标需要设计新的先进材料，具有生物相容性，生物降解性和足够的机械性能等特征。我们的工作重点是开发一种新的可注射仿生复合材料，基于肽水凝胶和磷酸钙，目的是模仿天然骨组织的化学组成。Arg-Gly-Asp-grafted chitosan用于促进细胞粘附。采用差示扫描量热法、流变学分析、场发射扫描电子显微镜、傅里叶变换红外光谱和核磁共振等方法对复合水凝胶进行了表征。使用MG-63人成骨细胞系评估生物反应性。
The goal of supporting and directing tissue regeneration requires the design of new, advanced materials, with features like biocompatibility, biodegradability and adequate mechanical properties. Our work was focused on developing a new injectable biomimetic composite material, based on a peptidic hydrogel and calcium phosphates with the aim of mimicking the chemical composition of natural bone tissue. Arg-Gly-Asp-grafted chitosan was used to promote cell adhesion. The obtained composite hydrogel was characterized with differential scanning calorimetry measurements, rheological analysis, field emission scanning electron microscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance measurements. The biological responsiveness was assessed using the MG-63 human osteoblast cell line.