Comparative proteomic analysis of latex from Euphorbia kansui laticifers at different development stages with and without UV-B treatment via iTRAQ-coupled two-dimensional liquid chromatography-MS/MS
Comparative proteomic analysis of latex from Euphorbia kansui laticifers at different development stages with and without UV-B treatment via iTRAQ-coupled two-dimensional liquid chromatography-MS/MS
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通过 iTRAQ 耦合二维液相色谱-MS/MS 对经过和未经 UV-B 处理的不同发育阶段的甘遂乳胶乳进行比较蛋白质组学分析
DOI：
10.1071/fp19033
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发表时间：
2020
影响因子：
3
通讯作者：
Xia Cai
中科院分区：
文献类型：
--
作者：
Xueyan Zhao;Yue Zhang;Meng Wang;Xiaoai Fang;Xia Cai
大戟是中国的特有种，在中药中享有盛名。所有的部分。kansui的植物含有白色乳胶，这是被称为乳汁管的特殊细胞的原生质成分。乳胶。含有多种具有多种生物学功能的蛋白质。在本研究中，相对和绝对等压标记。采用定量（iTRAQ）和质谱技术结合western blot和实时荧光定量PCR分析。用于鉴定乳汁蛋白，分析乳汁管在不同发育阶段积累的差异蛋白。根据kansui E.转录组数据库和NCBI，有无UV-B处理的分期。大戟科RefSeq蛋白数据库。共鉴定出322种乳胶蛋白。蛋白酶体。亚基、泛素化蛋白、空泡ATP合成酶（v -ATP酶）和溶酶体酶减少，保持了酶的活性。乳汁管在早期发育阶段含量较高。这些结果提示泛素蛋白酶体。途径和溶酶体自噬途径参与了乳汁管细胞质的部分降解。在。萜类生物合成相关蛋白、14-3-3蛋白、v - atp酶和溶酶体酶均升高。UV-B处理表明，部分细胞质降解与次生代谢物呈正相关。甘穗乳汁虫发育过程中的合成。此外，UV-B辐射还能通过促进植物抗性的增强。甘菊乳汁管发育。这些信息为进一步探索甘穗乳汁菊的发育、萜类化合物的合成和调控提供了依据。
Euphorbia kansui Liou, an endemic species in China, is well-known in traditional Chinese medicine. All parts.of E. kansui contain white latex, which is the protoplasm constituent of specialised cells known as laticifers. The latex.contains many proteins with various biological functions. In the present study, isobaric tagging for relative and absolute.quantitation (iTRAQ) and MS technology combined with western blot and quantitative real-time PCR analysis were.used to identify latex proteins and analyse differentially accumulated proteins in laticifers at different development.stages, with and without UV-B treatment according to the E. kansui transcriptome database and the NCBI.Euphorbiaceae RefSeq protein database. A total of 322 latex proteins were successfully identified. Proteasome.subunits, ubiquitinated proteins, vacuolar ATP synthase (V-ATPase) and lysosomal enzymes decreased, keeping the.content at a higher level in laticifers in the early development stage. These results suggest that the ubiquitin–proteasome.pathway and the lysosome autophagy pathway were involved in the partial degradation of laticifer cytoplasm. In.addition, terpenoid biosynthesis-related proteins, 14–3–3 protein, V-ATPase and lysosomal enzymes increased under.UV-B treatment, which showed that partial cytoplasmic degradation is positively correlated with secondary metabolite.synthesis in the development of E. kansui laticifers. Besides, UV-B radiation can increase plant resistance by promoting.laticifer development in E. kansui. This information provides a basis for further exploration of E. kansui laticifer.development, and terpenoid synthesis and regulation.