Direct targeting of light signals to a promoter element-bound transcription factor
Direct targeting of light signals to a promoter element-bound transcription factor
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DOI：
10.1126/science.288.5467.859
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发表时间：
2000-05-05
期刊：
影响因子：
56.9
通讯作者：
Quail, PH
中科院分区：
文献类型：
--
作者：
Martínez-García, JF;Huq, E;Quail, PH
由光敏色素家族的光感受器感知的光信号通过一种未知机制转导至光响应基因。在此，我们表明碱性螺旋 - 环 - 螺旋转录因子PIF3特异性结合存在于各种光调节基因启动子中的G - 盒DNA序列基序，并且在光触发光感受器转化为其生物活性构象时，光敏色素B特异性地与结合在G - 盒上的PIF3可逆结合。我们认为光敏色素可能作为转录调节复合物中不可或缺的光可切换组分发挥作用，从而允许直接在靶基因启动子处对这种环境信号的变化进行持续和即时的感知。
Light signals perceived by the phytochrome family of sensory photoreceptors are transduced to photoresponsive genes by an unknown mechanism. Here, we show that the basic helix-loop-helix transcription factor PIF3 binds specifically to a G-box DNA-sequence motif present in various Light-regulated gene promoters, and that phytochrome B binds reversibly to G-box-bound PIF3 specifically upon light-triggered conversion of the photoreceptor to its biologically active conformer. We suggest that the phytochromes may function as integral Light-switchable components of transcriptional regulator complexes, permitting continuous and immediate sensing of changes in this environmental signal directly at target gene promoters.