Independent evolution of human immunodeficiency virus type 1 env V1/V2 and V4/V5 hypervariable regions during chronic infection
Independent evolution of human immunodeficiency virus type 1 env V1/V2 and V4/V5 hypervariable regions during chronic infection
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DOI：
10.1128/jvi.02554-06
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发表时间：
2007-05-01
影响因子：
5.4
通讯作者：
Swanstrom, Ronald
中科院分区：
文献类型：
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作者：
Harrington, Patrick R.;Nelson, Julie A. E.;Swanstrom, Ronald
使用DNA异源双链追踪分析，我们的特点是人类免疫缺陷病毒1型env V4/V5的遗传群体在多个血浆样本收集超过平均7个月，从24个慢性感染的人类受试者。我们在大多数受试者中观察到复杂和动态的V4/V5遗传群体。在研究过程中，V4/V5和V1/V2群体变化的比较表明，遗传群体的主要变化经常发生在一个地区，而不是另一个地区，这些观察结果在一个受试者中通过单基因组测序得到独立证实。这些结果表明，在慢性感染过程中，env的V1/V2和V4/V5区域通常独立进化。
Using DNA heteroduplex tracking assays, we characterized human immunodeficiency virus type 1 env V4/V5 genetic populations in multiple blood plasma samples collected over an average of 7 months from 24 chronically infected human subjects. We observed complex and dynamic V4/V5 genetic populations in most subjects. Comparisons of V4/V5 and V1/V2 population changes over the course of the study showed that major shifts in genetic populations frequently occurred in one region but not the other, and these observations were independently confirmed in one subject by single-genome sequencing. These results suggest that the V1/V2 and V4/V5 regions of env often evolve independently during chronic infection.