Thiohalobacter thiocyanaticus gen. nov., sp. nov., a moderately halophilic, sulfur-oxidizing gammaproteobacterium from hypersaline lakes, that utilizes thiocyanate.
Thiohalobacter thiocyanaticus gen. nov., sp. nov., a moderately halophilic, sulfur-oxidizing gammaproteobacterium from hypersaline lakes, that utilizes thiocyanate.
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DOI：
10.1099/ijs.0.012880-0
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发表时间：
2010-02
影响因子：
2.8
通讯作者：
D. Sorokin;O. Kovaleva;T. Tourova;G. Muyzer
中科院分区：
文献类型：
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作者：
D. Sorokin;O. Kovaleva;T. Tourova;G. Muyzer
中度嗜盐、专性化能自养、硫氧化细菌，命名为 HRh1(T) 菌株，是从西伯利亚西南部库伦达草原（俄罗斯）的高盐氯化物-硫酸盐湖的混合沉积物样品中获得的，采用硫氰酸盐作为底物在 1 M 氯化钠下进行需氧富集培养。分离物的细胞是短的、非运动的杆状体，具有革兰氏阴性类型的细胞壁。该细菌是一种专性需氧微生物，能够在硫氰酸盐和硫代硫酸盐的作用下进行化能自养生长。使用硫代硫酸盐时，它在 0.2-3.0 M（最佳 0.5 M）的 NaCl 浓度和 pH 6.3-8.0（最佳 pH 7.3-7.5）条件下生长。在硫氰酸盐上生长期间，氰酸盐被鉴定为中间体。主要的细胞脂肪酸是C(16:0)和C(18:1)omega7。基于 16S rRNA 基因测序的系统发育分析将该分离株作为一个独立谱系归入 Gammaproteobacteria 类，其中未分类的海洋硫氧化细菌作为最接近的可培养亲属（93% 序列相似性）。在菌株 HRh1(T) 中检测到与化能自养生物中发现的任何同源基因具有相对较低相似性的单个 cbbL 基因（编码自养 CO(2) 同化卡尔文-本森循环的关键酶）。在我们的表型和系统发育分析的基础上，建议嗜盐分离株代表一个新属和新种，即硫氰酸硫盐杆菌属。十一月，SP。十一月硫氰酸硫盐杆菌的模式菌株是HRh1(T)(＝DSM 21152(T)＝UNIQEM U249(T))。
A moderately halophilic, obligately chemolithoautotrophic, sulfur-oxidizing bacterium, designated strain HRh1(T), was obtained from mixed sediment samples from hypersaline chloride-sulfate lakes in the Kulunda Steppe, in south-western Siberia (Russia), using aerobic enrichment culture at 1 M NaCl with thiocyanate as substrate. Cells of the isolate were short, non-motile rods with a Gram-negative type of cell wall. The bacterium was an obligate aerobe capable of chemolithoautotrophic growth with thiocyanate and thiosulfate. With thiosulfate, it grew at NaCl concentrations of 0.2-3.0 M (optimum 0.5 M) and at pH 6.3-8.0 (optimum pH 7.3-7.5). During growth on thiocyanate, cyanate was identified as an intermediate. The dominant cellular fatty acids were C(16 : 0) and C(18 : 1)omega7. Phylogenetic analysis based on 16S rRNA gene sequencing placed the isolate in the class Gammaproteobacteria as an independent lineage, with an unclassified marine sulfur-oxidizing bacterium as the closest culturable relative (93 % sequence similarity). A single cbbL gene (coding for the key enzyme of the Calvin-Benson cycle of autotrophic CO(2) assimilation) with relatively low similarity to any homologous genes found in chemolithoautotrophs was detected in strain HRh1(T). On the basis of our phenotypic and phylogenetic analysis, the halophilic isolate is proposed to represent a new genus and novel species, Thiohalobacter thiocyanaticus gen. nov., sp. nov. The type strain of Thiohalobacter thiocyanaticus is HRh1(T) (=DSM 21152(T) =UNIQEM U249(T)).