Pseudomonas syringae pv. tomato hijacks the Arabidopsis abscisic acid signalling pathway to cause disease
Pseudomonas syringae pv. tomato hijacks the Arabidopsis abscisic acid signalling pathway to cause disease
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DOI：
10.1038/sj.emboj.7601575
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发表时间：
2007-03-07
期刊：
影响因子：
11.4
通讯作者：
Grant, Murray
中科院分区：
文献类型：
--
作者：
de Torres-Zabala, Marta;Truman, William;Grant, Murray
我们已经发现，一个主要的目标，由假单胞菌分泌的效应是脱落酸（阿坝）信号通路。微阵列数据确定了一组突出的效应诱导的基因与阿坝的生物合成，也响应这种植物激素。通过效应子传递上调的基因与ABA响应基因有42%的重叠，并且也是渗透胁迫和干旱诱导的网络的组成部分。受到强烈诱导的是NCED 3（编码阿坝生物合成的关键酶）和编码参与阿坝信号调节的2C型蛋白磷酸酶（PP 2Cs）的基因的脱落酸不敏感1（ABI 1）分支。修饰PP2C表达导致阿坝不敏感或过敏导致限制或增强细菌的繁殖，分别。在细菌定植过程中阿坝水平迅速增加。外源阿坝的应用增强易感性，而在阿坝生物合成突变体的殖民减少。细菌效应子AvrPtoB在植物中的表达修饰了宿主阿坝信号传导。我们的数据表明，一个主要的毒力策略是效应介导的操纵植物激素的稳态，从而导致抑制防御反应。
We have found that a major target for effectors secreted by Pseudomonas syringae is the abscisic acid (ABA) signalling pathway. Microarray data identified a prominent group of effector-induced genes that were associated with ABA biosynthesis and also responses to this plant hormone. Genes upregulated by effector delivery share a 42% overlap with ABA-responsive genes and are also components of networks induced by osmotic stress and drought. Strongly induced were NCED3, encoding a key enzyme of ABA biosynthesis, and the abscisic acid insensitive 1 (ABI1) clade of genes encoding protein phosphatases type 2C (PP2Cs) involved in the regulation of ABA signalling. Modification of PP2C expression resulting in ABA insensitivity or hypersensitivity led to restriction or enhanced multiplication of bacteria, respectively. Levels of ABA increased rapidly during bacterial colonisation. Exogenous ABA application enhanced susceptibility, whereas colonisation was reduced in an ABA biosynthetic mutant. Expression of the bacterial effector AvrPtoB in planta modified host ABA signalling. Our data suggest that a major virulence strategy is effector-mediated manipulation of plant hormone homeostasis, which leads to the suppression of defence responses.