L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis
L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis
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DOI：
10.1093/humrep/den044
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发表时间：
2008-05-01
影响因子：
6.1
通讯作者：
Hornung, D.
中科院分区：
文献类型：
--
作者：
Finas, D.;Huszar, M.;Hornung, D.
背景：子宫内膜异位症是一种良性进行性疾病，患病率较高。患有子宫内膜异位症的女性，尤其是非典型子宫内膜异位症患者，相较于无此疾病的女性，患卵巢癌的概率更高。L1细胞黏附分子（L1CAM）在卵巢癌和子宫内膜癌中过度表达，且与不良预后相关。在此，我们分析了L1CAM在子宫内膜异位症中的表达情况。
方法与结果：在我们对79例子宫内膜异位症患者和37例无此疾病患者的样本进行的研究中，我们发现子宫内膜异位症细胞系以及子宫内膜异位症女性的子宫内膜短期培养物在mRNA和蛋白质水平上均表达L1CAM。定量逆转录聚合酶链反应分析表明，与健康对照组相比，子宫内膜异位症患者的上皮细胞区室中L1CAM的表达水平显著更高（P = 0.0126）。通过免疫组化染色，31例卵巢子宫内膜异位症病变中有15例（48%）显示L1CAM阳性染色。在这15例L1CAM阳性样本中，13例为非典型子宫内膜异位症病变。子宫内膜异位症女性的上皮子宫内膜培养物的条件培养基中存在的可溶性L1能够刺激神经突生长，这在鸡神经节实验中得到了验证。
结论：我们提出L1CAM可通过增加神经支配和加重病情来促进子宫内膜异位症的发展。与正常子宫内膜异位症相比，非典型子宫内膜异位症中L1CAM的表达更高。
BACKGROUND: Endometriosis is a benign and progressive disease with a high prevalence. Women with endometriosis, especially with atypical endometriosis, have a higher probability for developing ovarian cancer compared with women without endometriosis. The L1 cell adhesion molecule (L1CAM) is over expressed in ovarian and endometrial carcinomas and is associated with a bad prognosis. Here, we have analysed L1CAM expression in endometriosis. METHODS AND RESULTS: In our study with the samples from 79 patients with, and 37 patients without, endometriosis, we found that endometriosis cell lines and short-term cultures of endometrium from women with endometriosis expressed L1CAM at the mRNA and protein level. Quantitative RT-PCR analysis showed that L1CAM was expressed at significantly higher level in the epithelial compartment from patients with endometriosis compared with healthy controls (P= 0.0126). By immunohistochemical staining, 15 of 31 ovarian endometriotic lesions (48%) were shown to have L1CAM-positive staining. Of these 15 L1CAM-positive samples, 13 were atypical endometriotic lesions. Soluble L1 present in the conditioned medium of epithelial endometrium cultures from women with endometriosis was able to stimulate neurite outgrowth as measured in a chicken ganglion assay. CONCLUSIONS: We propose that L1CAM could promote endometriosis development by increasing enervation and aggravation. L1CAM expression is higher in atypical endometriosis compared with normal endometriosis.