Gas-Phase Fluorination on PLA Improves Cell Adhesion and Spreading
Gas-Phase Fluorination on PLA Improves Cell Adhesion and Spreading
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DOI：
10.1021/acsomega.0c00126
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发表时间：
2020-03
期刊：
影响因子：
4.1
通讯作者：
Michaela Schroepfer;F. Junghans;Diana Voigt;M. Meyer;Anette Breier;G. Schulze-Tanzil;I. Prade
中科院分区：
文献类型：
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作者：
Michaela Schroepfer;F. Junghans;Diana Voigt;M. Meyer;Anette Breier;G. Schulze-Tanzil;I. Prade
对于功能组织的再生或创造，包括聚乳酸(PLA)在内的可生物降解材料是广泛首选的材料。材料表面的修饰是非常常见的，以改善细胞与材料的相互作用，从而支持生物结果。典型的方法包括用主要有害物质的湿化学处理或用等离子体功能化。在本研究中，采用气相氟化一步法对聚乳酸表面进行功能化处理。在L929成纤维细胞的细胞培养实验中，分析了生物相容性、细胞黏附、细胞铺展和增殖等生物学反应，并与表面性质的变化进行了关联。采用表面能和等电点测量、X射线光电子能谱和原子力显微镜等表面表征方法对聚乳酸进行了氟化处理。气相氟化会导致聚乳酸主链中C-F键的形成，从而导致向更亲水和更具极性的表面转移。略带负电荷的表面显著改善了细胞在聚乳酸上的黏附和铺展，即使在低氟含量的情况下也是如此。结果表明，这种改善的生物反应是蛋白质依赖的，而不是整合素依赖的。因此，气相氟化是一种在不损失细胞相容性的情况下提高细胞对生物材料表面的响应的有效技术。
For the regeneration or creation of functional tissues, biodegradable biomaterials including polylactic acid (PLA) are widely preferred. Modifications of the material surface are quite common to improve cell–material interactions and thereby support the biological outcome. Typical approaches include a wet chemical treatment with mostly hazardous substances or a functionalization with plasma. In the present study, gas-phase fluorination was applied to functionalize the PLA surfaces in a simple and one-step process. The biological response including biocompatibility, cell adhesion, cell spreading, and proliferation was analyzed in cell culture experiments with fibroblasts L929 and correlated with changes in the surface properties. Surface characterization methods including surface energy and isoelectric point measurements, X-ray photoelectron spectroscopy, and atomic force microscopy were applied to identify the effects of fluorination on PLA. Gas-phase fluorination causes the formation of C–F bonds in the PLA backbone, which induce a shift to a more hydrophilic and polar surface. The slightly negatively charged surface dramatically improves cell adhesion and spreading of cells on the PLA even with low fluorine content. The results indicate that this improved biological response is protein- but not integrin-dependent. Gas-phase fluorination is therefore an efficient technique to improve cellular response to biomaterial surfaces without losing cytocompatibility.