Quercetin oxidation by horseradish peroxidase: The effect of UV-B irradiation

Quercetin oxidation by horseradish peroxidase: The effect of UV-B irradiation
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辣根过氧化物酶氧化槲皮素:UV-B 照射的影响

DOI:
10.2298/hemind120607093s
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发表时间:
2013
期刊:
Chemical Industry
影响因子:
--
通讯作者:
B. Petronijevic
B. Petronijevic
中科院分区:
--
文献类型:
--
作者:
R. Savić;S. Stanojević;Z. Markovic;B. Petronijevic

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辣根过氧化物酶(HRP),一个高度研究的成员过氧化物酶 在许多其他生物活性中,已知家族催化 总的类黄酮和酚类底物的氧化,包括 槲皮素另一方面,槲皮素因其 抗氧化活性,在紫外线外部辐射的情况下, 由于它是一种优良的紫外线吸收剂,因此部分地以预防的方式存在。 因此,本研究的目的是研究槲皮素的氧化 在紫外强制降解条件下,即连续条件下,磷酸盐缓冲液中的HRP, 延长UV-B辐射。结果表明,当UV-B辐射时, 影响HRP的活性和槲皮素氧化的总速率, HRP,它可能有很小的影响,它对它的较长的UV-B-照射 周期(>30 min)。[鸣谢。这项工作得到了该部的支持 根据TR-34012号项目, 和OI-172044]
Horseradish peroxidase (HRP), a highly-investigated member of the peroxidase family has been known, among many other biological activities, to catalyze the oxidation of flavonoids and phenolic substrates overall, including quercetin. On the other hand, quercetin is very well known for its antioxidant activities, which in the case of UV external radiation is exibited partly in a preventive manner since it is an excellent UV-absorber. Therefore the aim of this investigation is to study quercetin oxidation by HRP in phosphate buffer under the conditions of UV-stress, i.e. continuous, prolonged UV-B irradiation. The results show that while UV-B irradiation affects the activity of HRP, and the overal rate of quercetin oxidation by HRP, it probably has very little effect on it for longer UV-B-irradiation periods (>30 min). [Acknowledgements. This work was supported by the Ministry of Education and Science of the Republic of Serbia under Project No.TR-34012 and OI-172044]
DOI: 10.1016/j.foodchem.2009.10.056
发表时间: 2010-06-01
期刊: FOOD CHEMISTRY
影响因子: 8.8
作者:
Aiamla-or, Sukanya;Kaewsuksaeng, Samak;Yamauchi, Naoki
通讯作者: Yamauchi, Naoki