FANCM Limits Meiotic Crossovers
FANCM Limits Meiotic Crossovers
复制标题
DOI：
10.1126/science.1220381
复制
发表时间：
2012-06-22
期刊：
影响因子：
56.9
通讯作者：
Mercier, Raphael
中科院分区：
文献类型：
--
作者：
Crismani, Wayne;Girard, Chloe;Mercier, Raphael
减数分裂交换（CO）的数量是严格控制在一个狭窄的范围内，尽管大量过剩的分子前体。限制CO的因素在很大程度上仍然未知。在这里，使用拟南芥的遗传筛选，我们确定了高度保守的FANCM解旋酶，这是在人类和酵母的基因组稳定性所需的，作为一个主要因素限制减数分裂CO的形成。与野生型相比，fancm突变体的CO频率增加了三倍。这些额外的CO不是来自野生型中占大多数CO的途径，而是来自另一个通常较小的途径。因此，FANCM是限制减数分裂CO数目上限的一个关键因素，它的操纵在植物育种中具有很大的前景。
The number of meiotic crossovers (COs) is tightly regulated within a narrow range, despite a large excess of molecular precursors. The factors that limit COs remain largely unknown. Here, using a genetic screen in Arabidopsis thaliana, we identified the highly conserved FANCM helicase, which is required for genome stability in humans and yeasts, as a major factor limiting meiotic CO formation. The fancm mutant has a threefold-increased CO frequency as compared to the wild type. These extra COs arise not from the pathway that accounts for most of the COs in wild type, but from an alternate, normally minor pathway. Thus, FANCM is a key factor imposing an upper limit on the number of meiotic COs, and its manipulation holds much promise for plant breeding.