B7-H1 enhances proliferation ability of gastric cancer stem-like cells as a receptor.
B7-H1 enhances proliferation ability of gastric cancer stem-like cells as a receptor.
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B7-H1作为受体增强胃癌干细胞样细胞的增殖能力
DOI：
10.3892/ol.2015.2949
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发表时间：
2015-04
期刊：
影响因子：
2.9
通讯作者：
Tao K
中科院分区：
文献类型：
--
作者：
Yang Y;Wu KE;Zhao E;Li W;Shi L;Xie G;Jiang B;Wang Y;Li R;Zhang P;Shuai X;Wang G;Tao K
癌症干细胞样细胞（CSC）是一种罕见的致瘤细胞群，在许多癌症类型中具有自我更新的能力。它们的存在被认为是肿瘤复发的关键因素。B7-H1是抑制性诱导共刺激分子（ICOS）的配体，其在多种人类癌症中广泛表达。ICOS作为T细胞上程序性死亡-1（PD-1）的配体，诱导癌细胞的免疫逃逸，并且还作为介导对癌细胞的抗凋亡作用的受体。然而，B7-H1在CSCs上的表达和功能尚不清楚。本研究收集胃癌标本，检测B7-H1在胃癌CSC中的表达。Ki 67是一种增殖标志物，与B7-H1−对应物相比，在B7-H1+ CSC中的表达率更高。胃癌细胞系SGC-7901在无血清培养条件下形成具有干细胞特性的球形细胞，并在γ-干扰素（IFN-γ）的刺激下表达B7-H1。重组PD-1在体内外激活B7-H1后，球形细胞的增殖能力增强。这种影响可以通过中和B7-H1来消除。总之，B7-H1可以作为CSC的刺激受体，并诱导CSC增殖。阻断CSC上的B7-H1可能具有治疗胃癌的治疗潜力。
Cancer stem-like cells (CSCs) are a rare tumorigenic population with the ability to self-renew in numerous cancer types. Their existence is considered a pivotal contributor to tumor recurrence. B7-H1 is a ligand of inhibitory inducible co-stimulator (ICOS) that is broadly expressed on various human cancers. ICOS acts as a ligand of programmed death-1 (PD-1) on T cells, induces the immune escape of cancer cells and also acts as a receptor mediating anti-apoptotic effects on cancer cells. However, the expression and function of B7-H1 on CSCs is not yet clear. In the present study, gastric cancer samples were collected and the B7-H1 expression in gastric cancer CSCs was detected. Ki67, a proliferation marker, was found to be expressed at a higher rate in B7-H1+ CSCs compared with the B7-H1− counterparts. SGC-7901 cells, a gastric cancer cell line, were cultured in serum-free medium to form sphere cells that possessed stem cell characteristics and could express B7-H1 with the stimulation of interferon-γ. The proliferative ability of sphere cells was enhanced following B7-H1 activation with recombinant PD-1 in vivo and in vitro. This effect could be eliminated by neutralizing B7-H1. Overall, B7-H1 can act as a stimulating receptor for CSCs, and induce CSC proliferation. Blocking B7-H1 on CSCs may possess therapeutic potential for treating gastric cancer.
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影响因子：
10.1
作者：
Wang, Lijuan;Qian, Jianfei;Cai, Zhen
通讯作者：
Cai, Zhen
影响因子：
2.4
作者：
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通讯作者：
Leonard, JP
影响因子：
32.4
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影响因子：
2.5
作者：
Wu, Changping;Zhu, Yibei;Xu, Ning
通讯作者：
Xu, Ning
影响因子：
4.4
作者：
Kim, Yeong Seok;Park, Ga Bin;Hur, Dae Young
通讯作者：
Hur, Dae Young