A trehalose 6-phosphate synthase gene of the hemocytes of the blue crab, Callinectes sapidus: cloning, the expression, its enzyme activity and relationship to hemolymph trehalose levels.
A trehalose 6-phosphate synthase gene of the hemocytes of the blue crab, Callinectes sapidus: cloning, the expression, its enzyme activity and relationship to hemolymph trehalose levels.
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DOI：
10.1186/1746-1448-4-18
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发表时间：
2008-12-12
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影响因子：
--
通讯作者：
Chung JS
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文献类型：
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作者：
Chung JS
外胚层中的海藻糖在极端环境条件下的能量代谢和保护中起作用。我们的结构特征的海藻糖6-磷酸合酶（TPS）从血细胞的青蟹，Callinectes sapidus。C.与昆虫TPS一样，sapidus Hemo TPS（CasHemoTPS）编码TPS和海藻糖磷酸磷酸酶结构域。海藻糖似乎是一种主要的糖，因为它在血细胞和血淋巴中的水平高于葡萄糖。在脂多糖攻击后24小时测定HemoTPS表达、血细胞中TPS酶活性和血淋巴海藻糖水平的增加，表明CasHemoTPS的TPS和TPP结构域都是活性和功能性的。TPS基因在C. sapidus，暗示多个生物合成位点。在蜕皮周期中，血细胞中TPS活性与血淋巴海藻糖水平之间存在相关性。本研究首次证明了在红细胞中存在海藻糖，在组织中存在TPS。sapidus并暗示其在能量代谢和生理适应中的功能作用。
Trehalose in ectoderms functions in energy metabolism and protection in extreme environmental conditions. We structurally characterized trehalose 6-phosphate synthase (TPS) from hemocytes of the blue crab, Callinectes sapidus. C. sapidus Hemo TPS (CasHemoTPS), like insect TPS, encodes both TPS and trehalose phosphate phosphatase domains. Trehalose seems to be a major sugar, as it shows higher levels than does glucose in hemocytes and hemolymph. Increases in HemoTPS expression, TPS enzyme activity in hemocytes, and hemolymph trehalose levels were determined 24 h after lipopolysaccharide challenge, suggesting that both TPS and TPP domains of CasHemoTPS are active and functional. The TPS gene has a wide tissue distribution in C. sapidus, suggesting multiple biosynthetic sites. A correlation between TPS activity in hemocytes and hemolymph trehalose levels was found during the molt cycle. The current study provides the first evidence of presence of trehalose in hemocytes and TPS in tissues of C. sapidus and implicates its functional role in energy metabolism and physiological adaptation.