Qualitative and quantitative study of wound healing processes in the coelenterate, Plexaurella fusifera:: Spatial, temporal, and environmental (light attenuation) influences
Qualitative and quantitative study of wound healing processes in the coelenterate, Plexaurella fusifera:: Spatial, temporal, and environmental (light attenuation) influences
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DOI：
10.1006/jipa.1999.4851
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发表时间：
1999-05-01
影响因子：
3.4
通讯作者：
Bigger, C
中科院分区：
文献类型：
--
作者：
Meszaros, A;Bigger, C
受伤后，加勒比海软珊瑚（Plexaurella fusifera）形成一个包含变形虫细胞和虫黄藻（一种光合内共生体）的上皮前沿。变形虫细胞可能负责挤压组织再生所必需的结缔组织纤维，虫黄藻可能提供修复的能量。本研究考察了时间、空间和环境（光衰减）对这种珊瑚周围愈合的影响，并定量证实了受伤区域阿米巴细胞浓度的增加。通过移除约4.5毫米的共生酶，在珊瑚小臂上做了一个伤口。在损伤后的指定时间收集样本进行大体形态学和组织学评估，其中在0.009 mm(3)的组织内定量变形虫细胞和虫黄藻浓度。总的来说，未受伤组织和受伤组织内的阿米巴细胞数量相似。然而，当计算每面积损伤组织的变形虫细胞数量时，与未损伤组织相比，距离伤口边缘0-0.5 mm处的变形虫细胞增加82.4%，而距离伤口边缘0- 2mm处的变形虫细胞较少。虫黄藻浓度的总体增加也发生在受伤的珊瑚中，但没有发现明显的时间、空间或与光相关的模式。因此，这项研究支持了一个猜想，即在伤口部位的阿米巴细胞积累是细胞从靠近伤口边缘的未受伤组织迁移到伤口边缘的结果。此外，这种运动与光衰减无关。在这项研究中，光被限制在70到545 μ E - s(-1) m（-2）之间，在闭合或细胞浓度方面对伤口愈合过程没有显著影响。(C) 1999学术出版社。
Following injury the Caribbean soft coral, Plexaurella fusifera, forms an epithelial front containing amoebocytes and zooxanthellae, a photosynthetic endo symbiont. Amoebocytes may be responsible for extruding the connective mesogleal fibers necessary for regeneration of tissue and zooxanthellae may provide the energy for repair. This study examined the effects of time, space, and environment (light attenuation) on around healing in this coral species and quantitatively confirmed the increase of amoebocyte concentrations in the injured area. A wound was made on coral brachlets by removing similar to 4.5 mm of coenenchyme. At assigned times after injury, samples were collected for gross morphological and histological evaluation, in which amoebocytes and zooxanthellae concentrations were quantified within 0.009 mm(3) of tissue. Overall amoebocyte numbers within uninjured and wounded tissue were similar. However, when numbers of amoebocytes per area of injured tissue were calculated and compared to those of uninjured tissue, 82.4% more amoebocytes occurred at distances 0-0.5 mm from the wound edge, while areas of tissue >2 mm from the wound edge were occupied by fewer amoebocytes. Overall increases in concentrations of zooxanthellae also occurred within wounded coral, but no apparent temporal, spatial, or light-related pattern was detected. Therefore, this study supports the conjecture that amoebocyte accumulation at a wound site is an effect of cells migrating from uninjured tissue adjacent to the wounded edge. In addition, this movement occurs regardless of light attenuation. Light, which in this study was confined to ranges between 70 and 545 mu E s(-1) m(-2), did not significantly affect the wound healing process in regard to either closure or cellular concentrations. (C) 1999 Academic Press.