Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography
Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography
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DOI：
10.3791/51041
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发表时间：
2014-02-01
影响因子：
1.2
通讯作者：
Liao, Ronglih
中科院分区：
文献类型：
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作者：
Cheng, Hui-Wen;Fisch, Sudeshna;Liao, Ronglih
新出现的临床数据支持这样的观点：右心室功能障碍对于心血管疾病和心力衰竭的发病机制至关重要(1-3)。此外，RV 在肺动脉高压 (PAH) 等肺部疾病中受到显着影响。此外，右心室对心脏病理学非常敏感，包括左心室 (LV) 功能障碍、瓣膜疾病或右心室梗塞 (4)。为了了解 RV 在心脏病发病机制中的作用，一种可靠且无创的方法来从结构和功能上了解 RV 是至关重要的。建立并验证了一种基于无创经胸超声心动图 (TTE) 的方法，用于监测成年小鼠 RV 结构和功能的动态变化。为了施加右心室压力，我们采用了肺动脉收缩 (PAC) 手术模型，并使用高频超声显微成像系统测量了 7 天期间的右心室反应。使用假手术小鼠作为对照。在轻度麻醉的小鼠中在基线（手术前）、第 0 天（手术后立即）、第 3 天和第 7 天（手术后）获取图像。使用软件离线分析数据。可在小鼠中一致获得的多个声窗（B、M 和彩色多普勒模式）允许对正常小鼠和后续小鼠的 RV 结构（包括 RV 壁厚度、舒张末期和收缩末期尺寸）和功能（面积变化分数、缩短分数、PA 峰值速度和峰值压力梯度）进行可靠且可重复的测量 PAC。使用这种方法，使用彩色多普勒模式实时准确测量 PAC 产生的压力梯度，并且与使用 Millar 高保真微尖导管进行的直接压力测量相当。总而言之，这些数据表明，使用超声心动图从各种补充视图获得的 RV 测量结果可靠、可重复，并且可以提供有关 RV 结构和功能的见解。这种方法将使人们更好地了解 RV 心功能障碍的作用。
Emerging clinical data support the notion that RV dysfunction is critical to the pathogenesis of cardiovascular disease and heart failure(1-3). Moreover, the RV is significantly affected in pulmonary diseases such as pulmonary artery hypertension (PAH). In addition, the RV is remarkably sensitive to cardiac pathologies, including left ventricular (LV) dysfunction, valvular disease or RV infarction(4). To understand the role of RV in the pathogenesis of cardiac diseases, a reliable and noninvasive method to access the RV structurally and functionally is essential.A noninvasive trans-thoracic echocardiography (TTE) based methodology was established and validated for monitoring dynamic changes in RV structure and function in adult mice. To impose RV stress, we employed a surgical model of pulmonary artery constriction (PAC) and measured the RV response over a 7-day period using a high-frequency ultrasound microimaging system. Sham operated mice were used as controls. Images were acquired in lightly anesthetized mice at baseline (before surgery), day 0 (immediately post-surgery), day 3, and day 7 (post-surgery). Data was analyzed offline using software.Several acoustic windows (B, M, and Color Doppler modes), which can be consistently obtained in mice, allowed for reliable and reproducible measurement of RV structure (including RV wall thickness, end-diastolic and end-systolic dimensions), and function (fractional area change, fractional shortening, PA peak velocity, and peak pressure gradient) in normal mice and following PAC.Using this method, the pressure-gradient resulting from PAC was accurately measured in real-time using Color Doppler mode and was comparable to direct pressure measurements performed with a Millar high-fidelity microtip catheter. Taken together, these data demonstrate that RV measurements obtained from various complimentary views using echocardiography are reliable, reproducible and can provide insights regarding RV structure and function. This method will enable a better understanding of the role of RV cardiac dysfunction.