Unique properties of R-type calcium currents in neocortical and neostriatal neurons
Unique properties of R-type calcium currents in neocortical and neostriatal neurons
复制标题
DOI：
10.1152/jn.2000.84.5.2225
复制
发表时间：
2000-11-01
影响因子：
2.5
通讯作者：
Surmeier, DJ
中科院分区：
文献类型：
--
作者：
Foehring, RC;Mermelstein, PG;Surmeier, DJ
Whole cell recordings from acutely dissociated neocortical pyramidal neurons and striatal medium spiny neurons exhibited a calcium-channel current resistant to known blockers of L-, N-, and P/Q-type Ca2+ channels. These R-type currents were characterized as high-voltage-activated (HVA) by their rapid deactivation kinetics, half-activation and half-inactivation voltages, and sensitivity to depolarized holding potentials. In both cell types, the R-type current activated at potentials relatively negative to other HVA currents in the same cell type and inactivated rapidly compared with the other HVA currents. The main difference between cell types was that R-type currents in neocortical pyramidal neurons inactivated at more negative potentials than R-type currents in medium spiny neurons. Ni2+ sensitivity was not diagnostic for R-type currents in either cell type. Single-cell RT-PCR revealed that both cell types expressed the alpha 1E mRNA, consistent with this subunit being associated with the R-type current.
