IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites
IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites
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DOI：
10.1016/s0165-2427(01)00413-5
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发表时间：
2002-01-15
影响因子：
1.8
通讯作者：
Valdez, RA
中科院分区：
文献类型：
--
作者：
Goff, WL;Johnson, WC;Valdez, RA
研究了干扰素 -γ和/或肿瘤坏死因子 -α作为牛巴贝斯虫裂殖子诱导一氧化氮（NO）产生的共刺激因子的需求，以及白细胞介素 -4和白细胞介素 -10的调节作用。纯化的牛巴贝斯虫裂殖子在不添加外源性干扰素 -γ和肿瘤坏死因子 -α的情况下，不会诱导未分化的单核细胞产生NO，除非从体内取出的单核细胞内源性地产生肿瘤坏死因子 -α。在后一种情况下，裂殖子刺激产生的NO仍然依赖于干扰素 -γ。没有证据表明单核细胞衍生的巨噬细胞（MDM）内源性合成肿瘤坏死因子 -α，并且单独的裂殖子不能诱导MDM中的肿瘤坏死因子 -α mRNA。然而，虽然裂殖子加干扰素 -γ诱导了MDM中肿瘤坏死因子 -α mRNA的表达，但没有产生NO。白细胞介素 -4和白细胞介素 -10均抑制裂殖子刺激的单核细胞中诱导型一氧化氮合酶（iNOS）的表达和NO的产生。（C）2002爱思唯尔科学出版社。保留所有权利。
The requirement for IFN-gamma and/or TNF-alpha as co-stimulants with Babesia bovis merozoites for nitric oxide (NO) production was examined, as well as the regulatory role of IL-4 and IL-10. Purified B. bovis merozoites did not induce the production of NO in undifferentiated monocytes without addition of exogenous IFN-gamma and TNF-alpha unless the monocytes taken ex vivo were producing TNF-alpha endogenously. Under the latter condition, the NO production resulting from merozoite stimulation remained IFN-gamma-dependent. There was no evidence for endogenous synthesis of TNF-alpha in monocyte-derived macrophages (MDM), and merozoites alone were incapable of inducing TNF-alpha mRNA in MDM. However, while merozoites plus IFN-gamma induced TNF-alpha mRNA expression in MDM, NO was not produced. Both IL-4 and IL-10 inhibited expression of iNOS and production of NO in merozoite-stimulated monocytes. (C) 2002 Elsevier Science B.V. All rights reserved.