3D-electrode architectures for enhanced direct bioelectrocatalysis of pyrroloquinoline quinone-dependent glucose dehydrogenase.
3D-electrode architectures for enhanced direct bioelectrocatalysis of pyrroloquinoline quinone-dependent glucose dehydrogenase.
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DOI：
10.1021/am5046026
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发表时间：
2014-10
影响因子：
9.5
通讯作者：
David Sarauli;K. Peters;Cheng-xi Xu;B. Schulz;D. Fattakhova‐Rohlfing;F. Lisdat
中科院分区：
文献类型：
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作者：
David Sarauli;K. Peters;Cheng-xi Xu;B. Schulz;D. Fattakhova‐Rohlfing;F. Lisdat
我们报告了用于高效直接生物电催化的复杂电极结构的制造。在开发的程序中，将磺化聚苯胺[聚（2-甲氧基苯胺-5-磺酸）-苯胺共聚物]中包埋的氧化还原酶吡咯喹啉醌依赖性葡萄糖脱氢酶固定在大孔氧化铟锡（macroITO）电极上。使用具有大表面积的3D导电支架与导电聚合物相结合，可以固定大量酶并使其与电极有效通信，从而增强直接生物电催化作用。在葡萄糖存在的情况下，所制造的生物电极在没有任何额外介体的情况下表现出异常高的直接生物电催化响应。与平面ITO电极相比，催化电流增加200倍以上。具有高长期稳定性（即使在存储 2 周后，电流响应仍保持在初始值的 90% 以上），具有导电聚合物的透明 3D MacroITO 结构为构建高效生物电子单元奠定了宝贵的基础，可用作释放葡萄糖和允许光学和电化学转导过程的指示剂。
We report on the fabrication of a complex electrode architecture for efficient direct bioelectrocatalysis. In the developed procedure, the redox enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase entrapped in a sulfonated polyaniline [poly(2-methoxyaniline-5-sulfonic acid)-co-aniline] was immobilized on macroporous indium tin oxide (macroITO) electrodes. The use of the 3D-conducting scaffold with a large surface area in combination with the conductive polymer enables immobilization of large amounts of enzyme and its efficient communication with the electrode, leading to enhanced direct bioelectrocatalysis. In the presence of glucose, the fabricated bioelectrodes show an exceptionally high direct bioelectrocatalytical response without any additional mediator. The catalytic current is increased more than 200-fold compared to planar ITO electrodes. Together with a high long-term stability (the current response is maintained for >90% of the initial value even after 2 weeks of storage), the transparent 3D macroITO structure with a conductive polymer represents a valuable basis for the construction of highly efficient bioelectronic units, which are useful as indicators for processes liberating glucose and allowing optical and electrochemical transduction.