Effects of Electroacupuncture Pretreatment on Inflammatory Response and Acute Kidney Injury in Endotoxaemic Rats
Effects of Electroacupuncture Pretreatment on Inflammatory Response and Acute Kidney Injury in Endotoxaemic Rats
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DOI：
10.1177/147323001103900521
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发表时间：
2011-09-01
影响因子：
1.6
通讯作者：
Li, W.
中科院分区：
文献类型：
--
作者：
Gu, G.;Zhang, Z.;Li, W.
本研究观察了电针预处理对内毒素（LPS）诱导的炎症反应和急性肾损伤的影响。电针大鼠足三里、内关穴或电刺激假穴30 min后，静脉注射LPS 5 mg/kg或生理盐水。注射LPS或生理盐水后240 min检测血浆细胞因子、血浆亚硝酸盐、肾诱导型一氧化氮合酶（iNOS）和核因子-κ B（NF-κ B）活性。检测各组大鼠血尿素氮（BUN）、肌酐（Cr）水平，并进行肾小管损伤评分。电针预处理显著降低LPS诱导的血浆肿瘤坏死因子-α和白细胞介素（IL）-1 β，升高血浆IL-10，降低血浆亚硝酸盐、肾iNOS和NF-κ B B活性。能显著降低LPS诱导的BUN、Cr和肾组织病理学评分。提示电针预处理可减轻LPS诱导的炎症反应，减轻急性肾损伤。
This study investigated the effect of electroacupuncture pretreatment on the lipopolysaccharide (LPS)-induced inflammatory response and on acute kidney injury in adult male pathogen-free Wistar rats. Rats received electroacupuncture at the Zusanli (ST36) and Neiguan (PC6) acupoints, or electrical stimulation at sham points, for 30 min before stimulation with either 5 mg/kg LPS intravenously or normal saline. Plasma cytokines, plasma nitrite, renal inducible nitric oxide synthase (iNOS) and nuclear factor kappa B (NF-kappa B) activity were assessed 240 min after LPS or normal saline injection. Blood urea nitrogen (BUN), creatinine (Cr) and histopathological score for renal tubular damage were also measured. Electroacupuncture pretreatment significantly decreased LPS-induced plasma tumour necrosis factor-a and interleukin (IL)-1 beta, increased plasma IL-10, and decreased plasma nitrite, renal iNOS and NF-kappa B activity. It also significantly decreased LPS-induced BUN, Cr and the renal histopathological score. These findings suggest that electroacupuncture pretreatment at the ST36 and PC6 acupoints attenuated the LPS-induced inflammatory response and mitigated acute kidney injury.