Increased endonuclease activity in an extract from mouse Ehrlich ascites tumor cells which had been treated with a partially purified interferon preparation: dependence of double-stranded RNA;.
Increased endonuclease activity in an extract from mouse Ehrlich ascites tumor cells which had been treated with a partially purified interferon preparation: dependence of double-stranded RNA;.
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用部分纯化的干扰素制剂处理的小鼠艾氏腹水肿瘤细胞提取物中核酸内切酶活性增加：双链RNA的依赖性；。
DOI：
10.1016/s0006-291x(76)80280-x
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发表时间：
1976
影响因子：
3.1
通讯作者：
P. Lengyel
中科院分区：
文献类型：
--
作者：
G. E. Brown;B. Lebleu;M. Kawakita;S. Shaila;G. Sen;P. Lengyel
呼肠孤病毒信使RNA在用部分纯化的干扰素制剂或干扰素诱导剂poly（I）·poly（C）处理的小鼠埃利希腹水肿瘤细胞的粗提取物（S30）中降解得比在未处理细胞的相应提取物中更快。更快的降解似乎是核酸内切酶作用的结果。核酸内切酶的活性依赖于反应混合物中双链呼肠孤病毒RNA的存在。
Reovirus messenger RNAs are degraded faster in crude extracts (S30) from mouse Ehrlich ascites tumor cells which have been treated with either a partially purified interferon preparation or the interferon inducer poly(I)·poly(C) than in corresponding extracts from untreated cells. The faster degradation appears to be a consequence of endonuclease action. The endonuclease activityinvitrodepends on the presence of double-stranded reo-virus RNA in the reaction mixture.