Estradiol and testosterone differently affect visceral pain-related behavioural responses in male and female rats
Estradiol and testosterone differently affect visceral pain-related behavioural responses in male and female rats
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DOI：
10.1016/j.ejpain.2009.10.016
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发表时间：
2010-07-01
影响因子：
3.6
通讯作者：
Giamberardino, Maria Adele
中科院分区：
文献类型：
--
作者：
Aloisi, Anna Maria;Affaitati, Giannapia;Giamberardino, Maria Adele
在疼痛研究中，性别差异的存在是众所周知的，女性受试者在许多慢性疼痛病症中更容易受到影响；然而，潜在的机制和性腺激素的参与仍然存在争议。这项研究评估了经过验证的人工结石大鼠模型的内脏疼痛以及雌二醇和睾酮给药的效果。成年雄性和雌性大鼠被分成几组，并在手术前 2 天开始用一种物质或油（媒介物）治疗 5 天，其中一半接受人工结石（Stone），一半仅接受假手术（Sham）。记录动物行为（输尿管危象、频率和持续时间）72小时；在观察期结束时测定所有组中雌二醇和睾酮的血浆水平。手术后，只有Stone大鼠表现出输尿管疼痛危象，油处理组存在显着的性别差异，其中雌性危象的数量和持续时间高于雄性。这种差异在雌二醇治疗组中不存在，其中仅女性的输尿管危象减少，而睾酮治疗对任一性别均没有影响。两性的雌二醇和睾酮血浆水平均受到治疗的影响。这些结果证实，同样在这种内脏疼痛模型中，女性比男性经历更多的疼痛；此外，他们还表明，雌二醇（而非睾酮）的超生理水平仅对女性具有镇痛作用。建议并讨论了性腺激素的剂量和性别依赖性功效。 (C) 2009 年欧洲联合会国际疼痛研究协会分会。由爱思唯尔有限公司出版。保留所有权利。
In the study of pain, the presence of sex differences is well known, with female subjects being more affected in a number of chronic painful conditions; however, the underlying mechanisms and the involvement of gonadal hormones, are still controversial. This study evaluated visceral pain in a validated rat model of artificial calculosis and the effects of estradiol and testosterone administration. Adult male and female rats were divided into groups and treated with one of the substances or Oil (vehicle) for 5 days, starting 2 days before surgery, with half receiving an artificial calculosis (Stone) and half only a sham (Sham) procedure. The animals' behaviour (ureteral crises, frequency and duration) were recorded for 72 h; estradiol and testosterone plasma levels were determined in all groups at the end of the observation period. After surgery, only Stone rats showed ureteral pain crises, with a significant sex difference in the Oil-treated groups in which the number and duration of crises were higher in females than in males. This difference was not present in the estradiol-treated groups in which ureteral crises were decreased only in females while testosterone treatment had no effect in either sex. Estradiol and testosterone plasma levels were affected by treatments in both sexes. These results confirm that, also in this model of visceral pain, females experience more pain than males; moreover, they show that supraphysiological levels of estradiol, but not of testosterone, are analgesic only in females. A dose and sex-dependent efficacy of gonadal hormones is suggested and discussed. (C) 2009 European Federation of International Association for the Study of Pain Chapters. Published by Elsevier Ltd. All rights reserved.