Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined Essential 8 Medium conditions.
Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined Essential 8 Medium conditions.
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DOI：
10.1007/978-1-62703-348-0_6
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发表时间：
2013-01-01
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影响因子：
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通讯作者：
Vemuri, Mohan C
中科院分区：
文献类型：
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作者：
Fontes, Andrew;Macarthur, Chad C;Vemuri, Mohan C
人诱导多能干细胞（iPSC）因其固有的分化成三个胚层所有细胞类型的能力而成为再生医学的重要潜在细胞来源。采用非整合重编程方法并在完全不含动物蛋白的培养条件下生成 iPSC 将是此类再生和细胞治疗应用的理想选择。在这里，我们描述了一种使用游离 iPSC 重编程载体生成非整合 iPSC 的方法。
Human-induced pluripotent stem cells (iPSCs) are an important potential source of cells for regenerative medicine due to their inherent ability to differentiate into all cell types of the three germ layers. Generation of iPSCs with a non-integrating reprogramming method and in culture conditions that are completely absent of animal proteins will be ideal for such regenerative and cell therapy applications. Here we describe a method to generate non-integrating iPSCs using the Episomal iPSC Reprogramming Vectors.