Elevated levels of prostaglandin E2 in the liver of rats fed a choline deficient diet: possible involvement in liver tumor promotion.
Elevated levels of prostaglandin E2 in the liver of rats fed a choline deficient diet: possible involvement in liver tumor promotion.
复制标题
饲喂缺乏胆碱饮食的大鼠肝脏中前列腺素 E2 水平升高：可能参与肝脏肿瘤的促进。
DOI：
10.1016/0304-3835(89)90020-7
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发表时间：
1989
期刊：
影响因子：
9.7
通讯作者：
Shinozuka,H
中科院分区：
文献类型：
--
作者：
Gupta,C;Banks,M;Shinozuka,H
研究了饲喂缺乏胆碱（CD）饮食（一种有效的促进肝脏肿瘤的方案）对雄性斯普拉格-道利大鼠肝脏中前列腺素代谢的影响。在肝脏酶改变病灶诱导的短期测定中，使用降血脂过氧化物酶体增殖剂和前列腺素代谢调节剂（例如吲哚美辛和鲱鱼油）检查了这种改变可能的生物学意义。 CD饮食喂养10-30天后，会导致肝脏中前列腺素E2(PGE2)水平增加2-2.5倍，而降血脂过氧化物酶体增殖剂4-氯-6(2,3-xylidino)嘧啶硫基(N-羟乙基)-乙酰胺(BR93l)和邻苯二甲酸二(2-乙基己基)-邻苯二甲酸酯(DEHP)则显着降低了前列腺素E2(PGE2)水平。该代谢物的水平。在 CD 饮食中添加 BR93l 或吲哚美辛可抑制饮食引起的 PGE2 升高，并且用鲱鱼油替代 CD 饮食中的脂肪也具有相同的效果。此外，在饮食促进8周后，在CD饮食中添加吲哚美辛和鲱鱼油都抑制了用单剂量二乙基亚硝胺启动的大鼠肝脏中γ-谷氨酰转肽酶阳性肝细胞灶的诱导。结果表明，前列腺素代谢的改变可能与 CD 饮食的肝脏肿瘤促进作用有关。
The effect of feeding a choline deficient (CD) diet, an efficient liver tumor promoting regimen, on the prostaglandin metabolism in the liver of male Sprague-Dawley rats was investigated. The possible biological significance of the alteration was examined using hypolipidemic peroxisome proliferators and modifiers of prostaglandin metabolism such as indomethacin and menhaden oil in the short term assay of the induction of enzyme altered foci in the liver. A CD diet, when fed for 10–30 days, induced 2–2.5· times increases in the levels of prostaglandin E2(PGE2) in the liver, while the hypolipidemic peroxisome proliferators, 4-chloro-6(2,3-xylidino) pyrimidinylthio(N-hydroxyethyl)-acetamide (BR93l) and di(2-ethylhexyl)-phthalate (DEHP), markedly reduced the levels of this metabolite. The addition of BR93l or indomethacin to a CD diet suppressed the diet-induced elevations of PGE2and a substitution of fats in a CD diet with menhaden oil had the same effect. Furthermore, both indomethacin and menhaden oil added to a CD diet suppressed the induction of γ-glutamyltranspeptidase positive hepatocyte foci in the liver of rats initiated with a single dose of diethylnitrosamine after 8 weeks of the dietary promotion. The results suggest that altered prostaglandin metabolism may be involved in the liver tumor promoting effect of a CD diet.