Tissue Culture Propagation of Acer ×freemanii Using Thidiazuron to Stimulate Shoot Tip Proliferation
Tissue Culture Propagation of Acer ×freemanii Using Thidiazuron to Stimulate Shoot Tip Proliferation
复制标题
使用噻苯隆刺激茎尖增殖的 Acer ×freemanii 组织培养繁殖
DOI：
10.21273/hortsci.21.5.1209
复制
发表时间：
1986
期刊：
影响因子：
1.9
通讯作者：
M. Meyer
中科院分区：
文献类型：
--
作者：
H. R. Kerns;M. Meyer
用55-60年生的树体作接穗和插条，繁殖了两个槭×费氏槭天然杂种无性系。将繁殖体的茎尖消毒后接种在改良的LS培养基上进行离体培养。芽的伸长、生根和芽基部愈伤组织的形成取决于所用生长调节剂的浓度。典型培养芽的下部发育良好的节可以分离和单独培养，以获得缓慢增加的繁殖体。当茎尖转移到含有0.01-0.05 μM N-苯基-N '-1，2，3-噻二唑-5-基脲（thidiazuron）的培养基上时，芽开始增殖，每隔6周可分化成3-10个新芽，用于进一步增殖或生根。将生根的芽转移到温室中进一步生长，随后转移到田间。
Two clones of natural hybrids Acer × freemanii were propagated as scions and cuttings from trees 55-60 years old. Shoot tips from propagules were disinfected and cultured on a modified Linsmeier-Skoog (LS) medium in vitro. Elongation of shoots, rooting, and callus formation at the shoot base were dependent on the concentration of growth regulator used. The lower well-developed nodes of the typical cultured shoots could be separated and cultured individually to obtain a slow increase in propagules. When shoot tips were transferred to a medium with 0.01-0.05 μM N-phenyl-N'-l,2,3-thiadiazol-5-ylurea (thidiazuron), shoot proliferation was initiated, which could be separated into 3-10 new shoots at 6-week intervals for further proliferation or rooting. Rooted shoots were transferred to the greenhouse for further growth and subsequent transfer to the field.