Two distinct classes of prechondrogenic cell types in the embryonic limb bud.
Two distinct classes of prechondrogenic cell types in the embryonic limb bud.
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DOI：
10.1016/0012-1606(83)90063-5
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发表时间：
1983-05
影响因子：
2.7
通讯作者：
B. Swalla;E. M. Owens;T. Linsenmayer;Michael Solursh
中科院分区：
文献类型：
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作者：
B. Swalla;E. M. Owens;T. Linsenmayer;Michael Solursh
差异表现在软骨形成的潜力的细胞来源于鸡翅芽的远端和近端的一半，早在第23阶段，在出现明显的软骨分化。在高细胞密度培养物中，从第23或24阶段肢芽的远端部分获得的细胞在微团培养物中自发地成软骨。然而，从近端部分获得的细胞在其作为原分化软骨细胞的分化中受阻，因为这些细胞在微团培养物中产生可检测的II型胶原，但不能合成显著水平的软骨蛋白聚糖或积累将针对硫酸化糖胺聚糖染色的细胞外基质。通过加入1 mMdbcAMP或50 μg/ml抗坏血酸盐，或通过将近端细胞与少量远端细胞（1个远端细胞比10个近端细胞）混合，可以刺激近端肢芽细胞的这种培养物形成阿新蓝染色结节。这些结果表明存在两个不同的阶段之间的prechondrogenic间充质细胞。早期阶段似乎能够向近端细胞提供软骨形成刺激。
Differences are demonstrated in the chondrogenic potential of cells derived from the distal and proximal halves of chick wing buds from as early as stage 23, prior to the appearance of overt cartilage differentiation. In high cell density cultures, cells obtained from the distal portions of stage 23 or 24 limb buds are spontaneously chondrogenic in micromass cultures. Cells obtained from the proximal portions, however, become blocked in their differentiation as protodifferentiated cartilage cells, since these cells in micromass cultures make detectable type II collagen, but fail to synthesize significant levels of cartilage proteoglycan or to accumulate an extracellular matrix that will stain for sulfated glycosaminoglycans. Such cultures of proximal limb bud cells can be stimulated to form alcian blue staining nodules by the addition of 1 mMdbcAMP or 50 μg/ml ascorbate, or by mixing proximal cells with small numbers of distal cells (1 distal cell to 10 proximal cells). These results demonstrate the existence of two distinct stages among prechondrogenic mesenchyme cells. The earlier stage appears to be able to provide a chondrogenic stimulus to proximal cells.