BMP2-mimicking peptide modified with E7 coupling to calcined bovine bone enhanced bone regeneration associating with activation of the Runx2/SP7 signaling axis
BMP2-mimicking peptide modified with E7 coupling to calcined bovine bone enhanced bone regeneration associating with activation of the Runx2/SP7 signaling axis
复制标题
用 E7 修饰的 BMP2 模拟肽与锻烧牛骨偶联可增强与 Runx2/SP7 信号轴激活相关的骨再生
DOI：
10.1002/jbm.b.34368
复制
发表时间：
2020-01-01
影响因子：
3.4
通讯作者：
Yang, Guoli
中科院分区：
文献类型：
--
作者：
Xi, Yue;Miao, Xiaoyan;Yang, Guoli
商业骨替代物，如煅烧牛骨(CBB)，目前被广泛用作自体骨的替代品。然而，CBB缺乏骨诱导活性，仅作为天然骨形成的支架。为了解决这一问题，我们设计并制备了一种七谷氨酸(E7)修饰的BMP2模拟肽(7E)，并进行了一系列全面的物理表征和体内、体外研究，以评价其在颅骨缺损修复中的作用。结果表明，经E7修饰后的植骨材料上的多肽含量显著增加。值得注意的是，7E具有相对稳定和持久的释放，通过调节Runx2/SP7轴的表达，促进大鼠骨髓间充质干细胞(BMSCs)的成骨分化，并促进兔颅骨缺损的骨再生。综上所述，本研究制备的E7修饰的BMP2模拟肽与CBB复合的生物材料具有无免疫毒性、使用方便、成本低等优点，是一种新型的骨强化材料。(C)2019 Wiley期刊，Inc.生物材料资源B部分：APPL生物材料108B：80-93,2020。
Commercial bone substitute, such as calcined bovine bone (CBB), is currently extensively used as an alternative to autogenous bone. However, CBB lacks osteoinductivity and merely serves as a scaffold for native bone formation. To address this issue, we designed and prepared a heptaglutamate (E7)-modified BMP2-mimicking peptide (7E) and carried out a series of comprehensive physical characterizations and in vivo and in vitro studies to evaluate its role in the repair of cranial defects. The data elucidated that the amount of peptide anchoring to the bone graft materials was remarkably increased after modified with E7. Of note, 7E had a relatively stable and durable release, which promoted the osteogenic differentiation of rat derived bone marrow mesenchymal stem cells (BMSCs) and enhanced the bone regeneration of a rabbit calvarial defect by regulating the expression of the Runx2/SP7 axis. In summary, the composite biomaterials incorporating the E7-modified BMP2-mimicking peptide and CBB prepared in this study is a novel bone augmentation material with the merits of non-immunotoxicity, convenience, and low cost. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:80-93, 2020.