The CWI pathway is activated through high hydrostatic pressure, enhancing glycerol efflux via the aquaglyceroporin Fps1 in<i>Saccharomyces cerevisiae</i>
The CWI pathway is activated through high hydrostatic pressure, enhancing glycerol efflux via the aquaglyceroporin Fps1 in<i>Saccharomyces cerevisiae</i>
复制标题
CWI 途径通过高静水压激活，通过<i>酿酒酵母</i>中的水甘油孔蛋白 Fps1 增强甘油流出
DOI：
10.1101/2022.11.15.516693
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Abe Fumiyoshi
中科院分区：
文献类型：
--
作者：
Mochizuki Takahiro;Tanigawa Toshiki;Shindo Seiya;Suematsu Momoka;Oguchi Yuki;Mioka Tetsuo;Kato Yusuke;Fujiyama Mina;Hatano Eri;Yamaguchi Masashi;Chibana Hiroji;Abe Fumiyoshi
真菌细胞壁是真菌抵御各种外部胁迫（如渗透压变化、有害药物和机械损伤）的初始屏障。本研究探讨了酿酒酵母细胞壁完整性（CWI）信号通路在高静水压反应中的作用。我们展示了跨膜机械传感器WSC 1和水甘油孔蛋白Fps1在高压下维持细胞生长的一般机制中的作用。在25 MPa下促进水流入细胞，如通过细胞体积的增加和质膜eisosome结构的损失所证明的，促进了Wsc1（CWI途径的活化剂）的活化。下游的丝裂原活化蛋白激酶Slt2在25 MPa下过度磷酸化。甘油流出通过Fps1磷酸化增加，Fps1磷酸化由CWI途径的下游组分启动，并有助于在高压下降低细胞内渗透压。通过完善的CWI途径阐明适应高压的机制可能会转化为哺乳动物细胞，并为细胞机械感觉提供新的见解。
The fungal cell wall is the initial barrier for the fungi against diverse external stresses, such as osmolarity changes, harmful drugs, and mechanical injuries. This study explores the roles of osmoregulation and the cell wall integrity (CWI) pathway in response to high hydrostatic pressure in the yeastSaccharomyces cerevisiae. We demonstrate the roles of the transmembrane mechanosensor Wsc1 and aquaglyceroporin Fps1 in a general mechanism to maintain cell growth under high-pressure regimes. The promotion of water influx into cells at 25 MPa, as evident by an increase in cell volume and a loss of the plasma membrane eisosome structure, promotes the activation of Wsc1, an activator of the CWI pathway. The downstream mitogen-activated protein kinase Slt2 was hyperphosphorylated at 25 MPa. Glycerol efflux increases via Fps1 phosphorylation, which is initiated by downstream components of the CWI pathway and contributes to the reduction in intracellular osmolarity under high pressure. The elucidation of the mechanisms underlying adaption to high pressure through the well-established CWI pathway could potentially translate to mammalian cells and provide novel insights into cellular mechanosensation.